Development of an ELISA for chum salmon (Oncorhynchus keta) growth hormone

A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of Low levels of serum growth hormone (GH) in chum salmon (Oncorhynchus keta). The antiserum to GH (a-rsGH) was obtained from a rabbit immunized with recombinant chum salmon GH. The noncompetitive ELISA was performed by a sandwich method using a-rsGH rabbit IgG as the first antibody, its biotinylated Fab' fragment as the second antibody, and the avidin-biotin reaction for signal amplification. This assay could he run in 3 days and routinely detected GH at concentrations as low as 0.5 ng/ml. The development of an ELISA for Chi made possible quantification of serum GH levels. In this assay system, parallel dilution curves were obtained using purified chum salmon GH and GH's from several species of the genus Oncorhynchus. (C) 1997 Elsevier Science Inc.