Sexual transmission of HIV-1 isolate showing G→A hypermutation

被引:11
作者
Caride, E
Brindeiro, RM
Kallas, EG
de Sá, CAM
Eyer-Silva, WA
Machado, E
Tanuri, A
机构
[1] Univ Fed Rio de Janeiro, Mol Virol Lab, Dept Genet, BR-21944970 Rio De Janeiro, Brazil
[2] UFESP, Escola Paulista Med, Sao Paulo, Brazil
[3] Hosp Univ Gaffree & Guinle, Rio De Janeiro, Brazil
[4] UFRJ, Hosp Univ Clementino Fraga Filho, Rio De Janeiro, Brazil
关键词
HIV-1; G -> A hypermutation; protease; reverse transcriptase; HAART; CD4+T cells; oligoclonal expansion;
D O I
10.1016/S1386-6532(01)00218-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Retroviral genomes with a high frequency of G --> A mutations are thought to originate during reverse transcription (RT). Here we present a case report of an AIDS patient infected with a subtype F variant where extensive G --> A hypermutation (G --> A Hypm) sequences were found in the protease gene. This patient was failing HAART at the time the hypermutation was found. These sequences were basically encountered in the proviral compartment on two occasions and were persistently absent in the plasma viral population. The patient's viral genotype showed several mutations related to antiretroviral drug resistance in RT (T69N, N184V, T215F, K219Q) and protease (M36I, G48V, I54V, T63L, V82A) genes. The drug regimen was changed and the viral load dropped 0.9 Logs and CD4 count increased by 200 cells/ml. The hypermutation was not found any more in a 1-year follow up. The patient's wife was infected with a similar virus strain and G A Hypm sequences were also detected in the RT gene. This is the first report of sexual transmitted G --> A Hypermutation in HIV-1 and suggest that this phenomenon can be genetically coded by the viral RT molecule. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:179 / 189
页数:11
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