Results of in vitro thermotherapy of apple cultivars

被引：54

作者：

Paprstein, Frantisek ^{[1
]}

Sedlak, Jiri ^{[1
]}

Polak, Jaroslav ^{[2
]}

Svobodova, Ladislava ^{[1
]}

Hassan, Mohamed ^{[2
]}

Bryxiova, Marcela ^{[2
]}

机构：

[1] Res & Breeding Inst Pomol Holovousy, Horice 50801, Czech Republic

[2] Crop Res Inst, Div Plant Hlth, Prague 16106, Czech Republic

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2008年 / 94卷 / 03期

关键词：

certification; ELISA; PCR; virus detection; virus elimination; virus infection;

D O I：

10.1007/s11240-008-9342-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Apple cultivars 'Idared' and 'Sampion' were selected for application of in vitro thermotherapy at 39 +/- 0.5 degrees C. The presence of viruses in selected initial trees was detected by enzyme-linked immuno-sorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) before the beginning of thermotherapy. Cultivars 'Idared' and 'Sampion' were both infected with Apple chlorotic leaf spot virus (ACLSV) and with Apple stem pitting virus (ASPV). Cultivar 'Sampion' was moreover infected with Apple stem grooving virus (ASGV). Both apple cultivars were successfully multiplied in in vitro cultures on Murashige and Skoog (MS) medium with 1.5 mg l(-1) 6-benzylaminopurine (BAP). After the end of thermotherapy and transfer to ex vitro conditions, three clones of apple cultivar 'Idared' were free of both viruses (ACLSV, ASPV) according to repeated RT-PCR and ELISA. On the contrary, the same viruses (ACLSV, ASGV and ASPV) as in the initial field-grown trees were detected by RT-PCR in all four clones of cultivar 'Sampion'. Other sanitation and testing of apple cultivars will continue in the following years.

引用

页码：347 / 352

页数：6

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