Microrheology of keratin networks in cancer cells

被引:6
|
作者
Paust, T. [1 ]
Paschke, S. [2 ]
Beil, M. [3 ]
Marti, O. [1 ]
机构
[1] Univ Ulm, Dept Expt Phys, D-89069 Ulm, Germany
[2] Univ Ulm, Dept Surg, D-89075 Ulm, Germany
[3] Univ Ulm, Dept Med 1, D-89081 Ulm, Germany
关键词
INTERMEDIATE-FILAMENT NETWORKS; PARTICLE-TRACKING; 2-POINT MICRORHEOLOGY; MICROSCOPY; MIGRATION; MECHANICS; DYNAMICS; DISEASE; MODULI;
D O I
10.1088/1478-3975/10/6/065008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microrheology is a valuable tool to determine viscoelastic properties of polymer networks. For this purpose measurements with embedded tracer beads inside the extracted network of pancreatic cancer cells were performed. Observing the beads motion with a CCD-high-speed-camera leads to the dynamic shear modulus. The complex shear modulus is divided into real and imaginary parts which give insight into the mechanical properties of the cell. The dependency on the distance of the embedded beads to the rim of the nucleus shows a tendency for a deceasing storage modulus. We draw conclusions on the network topology of the keratin network types based on the mechanical behavior.
引用
收藏
页数:5
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