Probability of double-strand breaks in genome-sized DNA by γ-ray decreases markedly as the DNA concentration increases

被引：9

作者：

Shimobayashi, Shunsuke F. ^{[1
]}

Iwaki, Takafumi ^{[2
]}

Mori, Toshiaki ^{[3
]}

Yoshikawa, Kenichi ^{[1
,4
]}

机构：

[1] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto 6068502, Japan

[2] Kyoto Univ, Fukui Inst Fundamental Chem, Kyoto 6068103, Japan

[3] Osaka Prefecture Univ, Ctr Radiat Res, Sakai, Osaka 5998570, Japan

[4] Doshisha Univ, Fac Life & Med Sci, Kyoto 6100394, Japan

来源：

JOURNAL OF CHEMICAL PHYSICS | 2013年 / 138卷 / 17期

基金：

日本学术振兴会;

关键词：

SINGLE-MOLECULE OBSERVATION; HUMAN-CELLS; IONIZING-RADIATION; IRRADIATED DNA; REPAIR; DAMAGE; WATER; QUANTIFICATION; ELECTRONS; RADICALS;

D O I：

10.1063/1.4802993

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by gamma-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P-1, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs. (C) 2013 AIP Publishing LLC.

引用

页数：5

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