Sialic acid in the lipopolysaccharide of Haemophilus influenzae:: strain distribution, influence on serum resistance and structural characterization

被引：158
作者：
Hood, DW [1 ]
Makepeace, K [1 ]
Deadman, ME [1 ]
Rest, RF [1 ]
Thibault, P [1 ]
Martin, A [1 ]
Richards, JC [1 ]
Moxon, ER [1 ]
机构：
[1] Univ Oxford, Inst Mol Med, John Radcliffe Hosp, Dept Paediat,Mol Infect Dis Grp, Oxford OX3 9DS, England
来源：
MOLECULAR MICROBIOLOGY | 1999年 / 33卷 / 04期
关键词：
D O I：
10.1046/j.1365-2958.1999.01509.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A survey of Haemophilus influenzae strains indicated that around one-third of capsular strains and over two-thirds of non-typeable strains included sialic acid in their lipopolysaccharides (LPS). Mutation of the CMP-Neu5Ac synthetase gene (siaB) resulted in a sialylation-deficient phenotype. Isogenic pairs, wild type and siaB mutant df two non-typeable strains were used to demonstrate that sialic acid influences resistance to the killing effect of normal human serum but has little effect on attachment to, or invasion of, cultured human epithelial cells or neutrophils. We determine for the first time the site of attachment of sialic acid in the LPS of a non-typeable strain and report that a small proportion of glycoforms include two sialic acid residues in a disaccharide unit.
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页码：679 / 692
页数：14
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