Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method

被引：161499

作者：

Livak, KJ ^{[1
]}

Schmittgen, TD

机构：

[1] Washington State Univ, Dept Pharmaceut Sci, Pullman, WA 99164 USA

[2] Appl Biosyst Inc, Foster City, CA 94404 USA

来源：

METHODS | 2001年 / 25卷 / 04期

关键词：

reverse transcription polymerase chain reaction; quantitative polymerase chain reaction; relative quantification; real-time polymerase chain reaction; Taq Man;

D O I：

10.1006/meth.2001.1262

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-DeltaDeltaCr) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-DeltaDeltaCr) method. In addition, we present the derivation and applications of two variations of the 2(-DeltaDeltaCr) method that may be useful in the analysis of real-time, quantitative PCR data. (C) 2001 Elsevier science.

引用

页码：402 / 408

页数：7

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