Spatio-temporal correlation super-resolution optical fluctuation imaging

被引:7
作者
Purohit, A. [1 ]
Vandenberg, W. [2 ]
Dertinger, T. [3 ]
Woell, D. [1 ]
Dedecker, P. [2 ]
Enderlein, J. [3 ]
机构
[1] Rhein Westfal TH Aachen, Inst Phys Chem, Landoltweg 2, D-52074 Aachen, Germany
[2] Katholieke Univ Leuven, Dept Chem, Celestijnenlaan 200G, B-3001 Heverlee, Belgium
[3] Georg August Univ, Inst Phys Biophys 3, D-37077 Gottingen, Germany
关键词
RECONSTRUCTION MICROSCOPY; DIFFRACTION-LIMIT; RESOLUTION;
D O I
10.1209/0295-5075/125/20005
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
We describe a new concept of multimodal super-resolution imaging which combines the cumulant analysis from Super-resolution Optical Fluctuation Imaging (SOFT) with the imprinting of three-dimensional, spectral or other information into peculiar Point-Spread Function (PSF) patterns. This concept allows for encoding multidimensional or multimodal information into a single image plane and to extract this information by an appropriate spatio-temporal correlation analysis of emitter fluctuations. Here, we develop the general theory of this concept, and present proof-of-principle experiments of three-dimensional super-resolution imaging. Copyright (C) EPLA, 2019
引用
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页数:6
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共 27 条
  • [1] Three-dimensional super-resolution and localization of dense clusters of single molecules
    Barsic, Anthony
    Grover, Ginni
    Piestun, Rafael
    [J]. SCIENTIFIC REPORTS, 2014, 4
  • [2] Imaging intracellular fluorescent proteins at nanometer resolution
    Betzig, Eric
    Patterson, George H.
    Sougrat, Rachid
    Lindwasser, O. Wolf
    Olenych, Scott
    Bonifacino, Juan S.
    Davidson, Michael W.
    Lippincott-Schwartz, Jennifer
    Hess, Harald F.
    [J]. SCIENCE, 2006, 313 (5793) : 1642 - 1645
  • [3] Widely accessible method for superresolution fluorescence imaging of living systems
    Dedecker, Peter
    Mo, Gary C. H.
    Dertinger, Thomas
    Zhang, Jin
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (27) : 10909 - 10914
  • [4] Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)
    Dertinger, T.
    Colyer, R.
    Iyer, G.
    Weiss, S.
    Enderlein, J.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (52) : 22287 - 22292
  • [5] Dertinger Thomas, 2012, Opt Nanoscopy, V1, P2
  • [6] Superresolution Optical Fluctuation Imaging (SOFI)
    Dertinger, Thomas
    Colyer, Ryan
    Vogel, Robert
    Heilemann, Mike
    Sauer, Markus
    Enderlein, Joerg
    Weiss, Shimon
    [J]. NANO-BIOTECHNOLOGY FOR BIOMEDICAL AND DIAGNOSTIC RESEARCH, 2012, 733 : 17 - 21
  • [7] Superresolution Optical Fluctuation Imaging with Organic Dyes
    Dertinger, Thomas
    Heilemann, Mike
    Vogel, Robert
    Sauer, Markus
    Weiss, Shimon
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (49) : 9441 - 9443
  • [8] Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)
    Dertinger, Thomas
    Colyer, Ryan
    Vogel, Robert
    Enderlein, Joerg
    Weiss, Shimon
    [J]. OPTICS EXPRESS, 2010, 18 (18): : 18875 - 18885
  • [9] Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI
    Duwe, S.
    Vandenberg, W.
    Dedecker, P.
    [J]. CHEMICAL COMMUNICATIONS, 2017, 53 (53) : 7242 - 7245
  • [10] Geissbueler S., 2012, OPT NANOSCOPY, V1, P4, DOI DOI 10.1186/2192-2853-1-4