Blue Light-Mediated Manipulation of Transcription Factor Activity In Vivo

被引：24

作者：

Masuda, Shinji ^{[1
,2
]}

Nakatani, Yuki ^{[3
]}

Ren, Shukun ^{[1
]}

Tanaka, Mikiko ^{[3
]}

机构：

[1] Tokyo Inst Technol, Ctr Biol Resources & Informat, Yokohama, Kanagawa 2268501, Japan

[2] Tokyo Inst Technol, Earth Life Sci Inst, Tokyo 1528550, Japan

[3] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Yokohama, Kanagawa 2268501, Japan

来源：

ACS CHEMICAL BIOLOGY | 2013年 / 8卷 / 12期

基金：

日本科学技术振兴机构;

关键词：

GENE-EXPRESSION; SPATIOTEMPORAL CONTROL; PROTEIN INTERACTIONS; SIGNAL-TRANSDUCTION; ACTIVATION; INDUCTION; NETWORK; COMPLEX;

D O I：

10.1021/cb400174d

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a novel technique for manipulating the activity of transcription factors with blue light (termed "PICCORO") using the bacterial BLUF-type photoreceptor protein PixD. The chimeric dominant-negative T-box transcription factor No Tail formed heterologous complexes with a PixD decamer in a light-dependent manner, and these complexes affected transcription repressor activity. When applied to zebrafish embryos, PICCORO permitted regulation of the activity of the mutant No Tail in response to 472-nm light provided by a light-emitting diode.

引用

页码：2649 / 2653

页数：5

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