Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia

被引：19
作者：
Gradissimo Oliveira, A. [1 ]
Delgado, C. [2 ]
Verdasca, N. [1 ]
Pista, A. [3 ]
机构：
[1] Natl Inst Hlth, Dept Infect Dis, Natl Reference Lab STI Human Papillomavirus & Gen, Lisbon, Portugal
[2] Univ Nova Lisboa, Dept Bioinformat & Computat Biol, Inst Tecnol Quim & Biol, P-2780156 Oeiras, Portugal
[3] Natl Inst Hlth, Dept Infect Dis, Lisbon, Portugal
来源：
CLINICAL MICROBIOLOGY AND INFECTION | 2013年 / 19卷 / 10期
关键词：
Cervical cancer; human papillomavirus 18; molecular marker; physical status; risk assessment; VIRAL INTEGRATION; HPV16; WOMEN; CANCER; EXPRESSION; LESIONS; GENE; LOAD;
D O I：
10.1111/1469-0691.12233
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p<0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.
引用
收藏
页码：E447 / E450
页数：4
相关论文
共 21 条
[1]   HPV16 and HPV18 in genital tumors: Significantly different levels of viral integration and correlation to tumor invasiveness [J].
Badaracco, G ;
Venuti, A ;
Sedati, A ;
Marcante, ML .
JOURNAL OF MEDICAL VIROLOGY, 2002, 67 (04) :574-582
[2]   Viral DNA load, physical status and E2/E6 ratio as markers to grade HPV16 positive women for high-grade cervical lesions [J].
Cricca, Monica ;
Morselli-Labate, Antonio Maria ;
Venturoli, Simona ;
Ambretti, Simone ;
Gentilomi, Giovanna Angela ;
Gallinella, Giorgio ;
Costa, Silvano ;
Musiani, Monica ;
Zerbini, Marialuisa .
GYNECOLOGIC ONCOLOGY, 2007, 106 (03) :549-557
[3]   Integrated human papillomavirus types 52 and 58 are infrequently found in cervical cancer, and high viral loads predict risk of cervical cancer [J].
Ho, Chih-Ming ;
Chien, Tsai-Yen ;
Huang, Shih-Hung ;
Lee, Bor-Heng ;
Chang, Shwu-Fen .
GYNECOLOGIC ONCOLOGY, 2006, 102 (01) :54-60
[4]   Integration of human papillomavirus type-16 and type-18 is a very early event in cervical carcinogenesis [J].
Huang, L. -W ;
Chao, S. -L ;
Lee, B. -H .
JOURNAL OF CLINICAL PATHOLOGY, 2008, 61 (05) :627-631
[5]   Physical state and expression of HPV DNA in benign and dysplastic cervical tissue: different levels of viral integration are correlated with lesion grade [J].
Hudelist, G ;
Manavi, M ;
Pischinger, KID ;
Watkins-Riedel, T ;
Singer, CF ;
Kubista, E ;
Czerwenk, KF .
GYNECOLOGIC ONCOLOGY, 2004, 92 (03) :873-880
[6]   Early integration of high copy HPV16 detectable in women with normal and low grade cervical cytology and histology [J].
Kulmala, SMA ;
Syrjänen, SM ;
Gyllensten, UB ;
Shabalova, IP ;
Petrovichev, N ;
Tosi, P ;
Syrjänen, KJ ;
Johansson, BC .
JOURNAL OF CLINICAL PATHOLOGY, 2006, 59 (05) :513-517
[7]   Mechanisms of human papillomavirus-induced oncogenesis [J].
Münger, K ;
Baldwin, A ;
Edwards, KM ;
Hayakawa, H ;
Nguyen, CL ;
Owens, M ;
Grace, M ;
Huh, K .
JOURNAL OF VIROLOGY, 2004, 78 (21) :11451-11460
[8]   HPV in the etiology of human cancer [J].
Munoz, Nubia ;
Castellsague, Xavier ;
de Gonzalez, Amy Berrington ;
Gissmann, Lutz .
VACCINE, 2006, 24 :1-10
[9]   Physical status and expression of HPV genes in cervical cancers [J].
Park, JS ;
Hwang, ES ;
Park, SN ;
Ahn, HK ;
Um, SJ ;
Kim, CJ ;
Kim, SJ ;
Namkoong, SE .
GYNECOLOGIC ONCOLOGY, 1997, 65 (01) :121-129
[10]   Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative real-time PCR technique [J].
Peitsaro, P ;
Johansson, B ;
Syrjänen, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :886-891
123