Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents

被引：6
作者：
Han, MS
Jung, SO
Kim, MJ
Kim, DH
机构：
[1] Pohang Univ Sci & Technol, Natl Res Lab Chirotechnol, Dept Chem, Div Mol & Life Sci, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Ctr Integrated Mol Syst, Pohang 790784, South Korea
来源：
JOURNAL OF ORGANIC CHEMISTRY | 2004年 / 69卷 / 08期
关键词：
D O I：
10.1021/jo040104i
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.
引用
收藏
页码：2853 / 2855
页数：3
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