Porcine DC-SIGN: Molecular cloning, gene structure, tissue distribution and binding characteristics

被引：57
作者：
Huang, Y. W. [1 ]
Dryman, B. A. [1 ]
Li, W. [1 ]
Meng, X. J. [1 ]
机构：
[1] Virginia Polytech Inst & State Univ, Coll Vet Med, Dept Biomed Sci & Pathobiol, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2009年 / 33卷 / 04期
关键词：
C-type lectins; DC-SIGN; Pig; Innate immunity; Cell adhesion; Dendritic cells; Mammals; Gene; C-TYPE LECTIN; RESPIRATORY SYNDROME VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; DENDRITIC CELL; ENDOTHELIAL-CELLS; LIGAND-BINDING; IMMUNE-SYSTEM; MOUSE DC; IN-VITRO; RECEPTOR;
D O I：
10.1016/j.dci.2008.09.010
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
DC-SIGN, a human C-type lectin, is involved in the transmission of many enveloped viruses. Here we report the cloning and characterization of the cDNA and gene encoding porcine DC-SIGN (pDC-SIGN). The full-length pDC-SIGN cDNA encodes a type 11 transmembrane protein of 240 amino acids. Phylogenetic analysis revealed that pDC-SIGN, together with bovine, canis and equine DC-SIGN, are more closely related to mouse SIGNR7 and SIGNR8 than to human DC-SIGN. pDC-SIGN has the same gene structure as bovine, canis DC-SIGN and mouse SIGNR8 with eight exons. pDC-SIGN mRNA expression was detected in pig spleen, thymus, lymph node, lung, bone marrow and muscles. pDC-SIGN protein was found to express on the surface of monocyte-derived macrophages and dendritic cells, alveolar macrophages, lymph node sinusoidal macrophage-like, dendritic-like and endothelial cells but not of monocytes, peripheral blood lymphocytes or lymph node lymphocytes. A BHK cell line stably expressing pDC-SIGN binds to human ICAM-3 and ICAM-2 immunoadhesins in a calcium-dependent manner, and enhances the transmission of porcine reproductive and respiratory syndrome virus (PRRSV) to target cells in trans. The results will help better understand the biological role(s) of DC-SIGN family in innate immunity during the evolutionary process. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：464 / 480
页数：17
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