Complex sputum microbial composition in patients with pulmonary tuberculosis

被引:73
作者
Cui, Zelin [1 ]
Zhou, Yuhua [1 ]
Li, Hong [2 ]
Zhang, Yan [1 ]
Zhang, Shulin [1 ]
Tang, Shenjie [2 ]
Guo, Xiaokui [1 ]
机构
[1] Shanghai Jiao Tong Univ, Dept Med Microbiol & Parasitol, Sch Med, Shanghai 200025, Peoples R China
[2] Tongji Univ, TB Ctr Diag & Treatment, Shanghai Pulm Hosp, Sch Med, Shanghai 200433, Peoples R China
来源
BMC MICROBIOLOGY | 2012年 / 12卷
基金
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词
Pulmonary tuberculosis; Sputum; Microbiota; Diversity; INFECTION; BACTERIA; PHENYLOBACTERIUM; CONSEQUENCES; COMMUNITIES; DISEASE; OBESITY; NOV;
D O I
10.1186/1471-2180-12-276
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: An increasing number of studies have implicated the microbiome in certain diseases, especially chronic diseases. In this study, the bacterial communities in the sputum of pulmonary tuberculosis patients were explored. Total DNA was extracted from sputum samples from 31 pulmonary tuberculosis patients and respiratory secretions of 24 healthy participants. The 16S rRNA V3 hyper-variable regions were amplified using bar-coded primers and pyro-sequenced using Roche 454 FLX. Results: The results showed that the microbiota in the sputum of pulmonary tuberculosis patients were more diverse than those of healthy participants (p<0.05). The sequences were classified into 24 phyla, all of which were found in pulmonary tuberculosis patients and 17 of which were found in healthy participants. Furthermore, many foreign bacteria, such as Stenotrophomonas, Cupriavidus, Pseudomonas, Thermus, Sphingomonas, Methylobacterium, Diaphorobacter, Comamonas, and Mobilicoccus, were unique to pulmonary tuberculosis patients. Conclusions: This study concluded that the microbial composition of the respiratory tract of pulmonary tuberculosis patients is more complicated than that of healthy participants, and many foreign bacteria were found in the sputum of pulmonary tuberculosis patients. The roles of these foreign bacteria in the onset or development of pulmonary tuberculosis shoud be considered by clinicians.
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页数:8
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