Different Region Analysis for Genotyping Yersinia pestis Isolates from China

被引：178

作者：

Li, Yanjun ^{[1
]}

Dai, Erhei ^{[1
]}

Cui, Yujun ^{[1
]}

Li, Min ^{[2
]}

Zhang, Yujiang ^{[3
]}

Wu, Mingshou ^{[4
]}

Zhou, Dongsheng ^{[1
]}

Guo, Zhaobiao ^{[1
]}

Dai, Xiang ^{[3
]}

Cui, Baizhong ^{[2
]}

Qi, Zhizhen ^{[2
]}

Wang, Zuyun ^{[2
]}

Wang, Hu ^{[2
]}

Dong, Xingqi ^{[4
]}

Song, Zhizhong ^{[4
]}

Zhai, Junhui ^{[1
]}

Song, Yajun ^{[1
]}

Yang, Ruifu ^{[1
]}

机构：

[1] Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Lab Analyt Microbiol, Beijing, Peoples R China

[2] Qinghai Inst Endem Dis Prevent & Control, Xining, Peoples R China

[3] Ctr Dis Control & Prevent, Xinjiang Uygur, Peoples R China

[4] Yunnan Inst Endem Dis Control & Prevent, Yunnan, Peoples R China

来源：

PLOS ONE | 2008年 / 3卷 / 05期

基金：

国家高技术研究发展计划(863计划);

关键词：

D O I：

10.1371/journal.pone.0002166

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: DFR (different region) analysis has been developed for typing Yesinia pestis in our previous study, and in this study, we extended this method by using 23 DFRs to investigate 909 Chinese Y. pestis strains for validating DFR-based genotyping method and better understanding adaptive microevolution of Y. pestis. Methodology/Principal Findings: On the basis of PCR and Bionumerics data analysis, 909 Y. pestis strains were genotyped into 32 genomovars according to their DFR profiles. New terms, Major genomovar and Minor genomovar, were coined for illustrating evolutionary relationship between Y. pestis strains from different plague foci and different hosts. In silico DFR profiling of the completed or draft genomes shed lights on the evolutionary scenario of Y. pestis from Y. pseudotuberculosis. Notably, several sequenced Y. pestis strains share the same DFR profiles with Chinese strains, providing data for revealing the global plague foci expansion. Conclusions/significance: Distribution of Y. pestis genomovars is plague focus-specific. Microevolution of biovar Orientalis was deduced according to DFR profiles. DFR analysis turns to be an efficient and inexpensive method to portrait the genome plasticity of Y. pestis based on horizontal gene transfer (HGT). DFR analysis can also be used as a tool in comparative and evolutionary genomic research for other bacteria with similar genome plasticity.

引用

页数：10

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