Knock-down of methylenetetrahydrofolate reductase reduces gastric cancer cell survival: An in vitro study

被引：7

作者：

Sun, Dan-Feng ^{[1
]}

Weng, Yu-Rong ^{[1
]}

Chen, Ying-Xuan ^{[1
]}

Lu, Rong ^{[1
]}

Wang, Xia ^{[1
]}

Fang, Jing-Yuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Shanghai Inst Digest Dis, Shanghai 200001, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2008年 / 32卷 / 08期

关键词：

Methionine dependence; Methylenetetrahydrofolate reductase; Gastric cancer; RNA interference;

D O I：

10.1016/j.cellbi.2008.03.018

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The present study aimed to investigate the effect of knocking-down methylenetetrahydrofolate reductase ( MTHFR) on the survival of the human gastric cancer cell line MKN45. Antisense and small interfering RNA ( siRNA) plasmids were used to target MTHFR in MKN45. Meanwhile, we also constructed a wild-type MTHFR plasmid to assess the effect of over-expression of this protein on cell viability. The knock-down of MTHFR decreased cell survival by approximately 30% compared to the control and resulted in cell cycle arrest at the G2 phase. These cells also had lower levels of c-myc compared to control cells, while over-expression of MTHFR increased cell proliferation and induced the down-regulation of p21(WAF1) and hMLH1. Inhibiting MTHFR with either antisense or siRNA decreases the viability of methionine-dependent transformed gastric cancer cells and suggests that MTHFR inhibition may be a novel anticancer approach. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：879 / 887

页数：9

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