Determination of Doxepin in human plasma using cloud-point extraction with high-performance liquid chromatography and ultraviolet detection

被引：11

作者：

Baezzat, Mohammad Reza ^{[1
]}

Banavand, Fatemeh ^{[1
]}

Tabandeh, Mahboobeh ^{[1
]}

Amiri, Ali Asghar ^{[2
]}

Pourghobadi, Rogheye ^{[1
]}

机构：

[1] Payame Noor Univ, Dept Chem, Tehran 193954697, Iran

[2] Islamic Azad Univ, Shiraz Branch, Fac Sci, Dept Appl Chem, Shiraz, Iran

来源：

JOURNAL OF SEPARATION SCIENCE | 2015年 / 38卷 / 18期

关键词：

Cloud-point extraction; Doxepin; High-performance liquid chromatography; Triton X-114; ATOMIC-ABSORPTION-SPECTROMETRY; PHASE MICROEXTRACTION; ANTIDEPRESSANT DRUGS; METABOLITES; SERUM; ANTIPSYCHOTICS; SURFACTANT; SAMPLES;

D O I：

10.1002/jssc.201500075

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new straightforward method based on cloud-point extraction has been developed, optimized, and validated for the determination of doxepin in human plasma by high-performance liquid chromatography separation and UV detection. The nonionic surfactant Triton X-114 was chosen as the extraction solvent. Chromatography separation was performed on a mu Bondapak(R) C-18 column (4.6 mm id x 300 mm, 3 mu m particle size), which was used for isocratic elution at a detection wavelength of 289 nm. Under the optimum conditions, the linear range of doxepin in human plasma was 0.1-0.9 mu g/mL. Also, the detection limit, preconcentration factor, and enrichment factor were 0.08 mu g/mL, 50, and 49.0, respectively.

引用

页码：3192 / 3197

页数：6

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