In vitro Evaluation of Mesoporous Carbonated Hydroxyapatite in MC3T3-E1 Osteoblast Cells

被引：18

作者：

Mohammad, Nur Farahiyah ^{[1
,2
]}

Othman, Radzali ^{[3
]}

Abdullah, Nurul Asma ^{[4
]}

Yeoh, Fei Yee ^{[1
]}

机构：

[1] Univ Sains Malaysia, Sch Mat & Mineral Resources Engn, Engn Campus, Nibong Tebal 14300, Penang, Malaysia

[2] Univ Malaysia Perlis, Sch Mechatron Engn, Biomed Elect Engn Programme, Pauh Putra Campus, Arau 02600, Perlis, Malaysia

[3] Univ Tekn Malaysia, Fac Mfg Engn, Durian Tunggal 76100, Melaka, Malaysia

[4] Univ Sains Malaysia, Sch Hlth Sci, Hlth Campus, Kubang Kerian 16150, Kelantan, Malaysia

来源：

5TH INTERNATIONAL CONFERENCE ON RECENT ADVANCES IN MATERIALS, MINERALS AND ENVIRONMENT (RAMM) & 2ND INTERNATIONAL POSTGRADUATE CONFERENCE ON MATERIALS, MINERAL AND POLYMER (MAMIP) | 2016年 / 19卷

关键词：

Mesoporous; Carbonated Hydroxyapatite; Cytotoxicity; Alkaline Phosphatase; HYDROTHERMAL SYNTHESIS; HYDROXYCARBONATE APATITE; HYDROXYAPATITE; DRUG; DELIVERY; FABRICATION; SURFACTANT; RECOMMENDATIONS; NANOPARTICLES; MICROSPHERES;

D O I：

10.1016/j.proche.2016.03.103

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Nanoporous bioceramic recently has gained attention as a drug storage and release host in the therapeutic application. Mesoporous carbonated hydroxyapatite (MCHA) is a nanoporous bioceramic with pore sized ranged between 2-50 nm. In order to use in drug delivery application, this material should demonstrate acceptable cytotoxic activity and osteoinductive response. Therefore, this study aimed to evaluate the effect of mesoporous carbonated hydroxyapatite on viability and alkaline phosphatase ( ALP) activity of MC3T3-E1 osteoblasts cell line. These parameters represent cytotoxicity level and osteinductivity capability of the materials. MC3T3-E1 cells were cultured in 25 mg/ml extraction of mesoporous carbonated hydroxyapatite ( meso-CHA) and non-porous CHA ( np-CHA) for up to 7 days for viability and 14 days for ALP test. Results indicate that meso-CHA shows better cytotoxicity properties compare to np-CHA. Intracellular ALP of cells treated with meso-CHA was higher than np-CHA. (C) 2016 The Authors. Published by Elsevier B.V.

引用

页码：259 / 266

页数：8

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