Boronic acid modified fiber optic SPR sensor and its application in saccharide detection

被引：32

作者：

Qian, Siyu ^{[1
]}

Liang, Yuzhang ^{[1
]}

Ma, Jie ^{[2
]}

Zhang, Yang ^{[1
]}

Zhao, Jianzhang ^{[2
]}

Peng, Wei ^{[1
]}

机构：

[1] Dalian Univ Technol, Coll Phys & Optoelect Engn, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 220卷

关键词：

Surface plasmon resonance; Fiber-optic sensor; Boronic acid; Saccharide; ARYLBORONIC ACIDS; SUGAR ACIDS; GLUCOSE; GLYCOPROTEIN; BINDING; ESTER;

D O I：

10.1016/j.snb.2015.06.107

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To extend the application of fiber-optic surface plasmon resonance (SPR) sensing technology, the chemical molecular, 2-(((6-aminohexyl)(benzyl)amino)methyl) phenyl boronic acid (ABA-PBA), is prepared as the recognition monolayer and immobilized on the fiber-optic SPR probe. The boronic acid moiety in ABA-PBA monolayer can covalently bond with 1, 2- or 1, 3- diols moiety of sugar. A monolayer of ABA-PBA is assembled on the SPR sensing region and confirmed by surface enhanced Raman scattering (SERS) technique. Spectrophotometric method is used to determine the pKa of ABA-PBA. The interaction between ABA-PBA monolayer and saccharide can induce the shift of resonance wavelength. By using the miniature fiber-optic SPR sensor, saccharide concentration can be monitored in real time. It shows good selectivity to fructose at low concentrations (10-50 mM). (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：1217 / 1223

页数：7

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