Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage

被引：3
|
作者：
Sato, Kae [1 ]
Kodama, Ayuki [1 ]
Kase, Chikako [1 ]
Hirakawa, Satoshi [2 ]
Ato, Manabu [3 ]
机构：
[1] Japan Womens Univ, Fac Sci, Dept Chem & Biol Sci, Bunkyo Ku, Tokyo 1128681, Japan
[2] Hamamatsu Univ Sch Med, Dept Dermatol, Hamamatsu, Shizuoka 4313192, Japan
[3] Natl Inst Infect Dis, Dept Immunol, Shinjuku Ku, Tokyo 1628640, Japan
来源：
ANALYTICAL SCIENCES | 2018年 / 34卷 / 03期
关键词：
Snake venom; cultured cell; vascular permeability; hemorrhage; HEMORRHAGIC METALLOPROTEINASE; ENDOTHELIAL PERMEABILITY; BOTHROPS-ASPER; INHIBITOR; PROTEASES; HABU; FLAVOVIRIDIS; BATIMASTAT; CULTURE; BARRIER;
D O I：
10.2116/analsci.34.323
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed a novel bioassay method for the detection of snake venom based on the permeability of endothelial cell monolayers cultured in Transwell cell culture inserts. This assay relies on the proteolytic degradation of capillary basement membrane proteins, a pathophysiological event that occurs due to snakebites in vivo. Transwell permeability assays with fluorescence measurements are advantageous with regard to ethical considerations for the use of animals. The assay time was reduced from 24 h for animal tests to 2 h, and many samples could be assayed easily.
引用
收藏
页码：323 / 327
页数：5
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