Biotin-decorated fluorescent silica nanoparticles with aggregation-induced emission characteristics: fabrication, cytotoxicity and biological applications

被引：84

作者：

Li, Min ^{[1
,2
,3
]}

Lam, Jacky W. Y. ^{[2
,3
]}

Mahtab, Faisal ^{[2
,3
]}

Chen, Sijie ^{[2
,3
]}

Zhang, Weijie ^{[1
]}

Hong, Yuning ^{[2
,3
]}

Xiong, Jun ^{[1
]}

Zheng, Qichang ^{[1
]}

Tang, Ben Zhong ^{[2
,3
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Hepatobiliary Surg, Wuhan 430022, Peoples R China

[2] Hong Kong Univ Sci & Technol, Inst Adv Study, Dept Chem, State Key Lab Mol Neurosci, Kowloon, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Div Biomed Engn, Kowloon, Hong Kong, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2013年 / 1卷 / 05期

基金：

美国国家科学基金会;

关键词：

OXIDATIVE STRESS; DRUG-DELIVERY; CELLULAR UPTAKE; IN-VIVO; EXPLORATION; APOPTOSIS; RECEPTOR; CELLS;

D O I：

10.1039/c2tb00155a

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Biotin-decorated fluorescent silica nanoparticles (FSNPs) were successfully fabricated by a sol-gel reaction of silole-functionalized siloxane followed by a sequential reaction with tetraethoxysilane, (3-aminopropyl) triethoxysilane and biotin. The FSNPs were uniformly sized, spherical in shape and monodispersed. While their silole precursor was non-emissive in solution, the suspension of the FSNPs emitted strong green light upon photoexcitation due to the aggregation-induced emission characteristics of the silole aggregates in the hybrid nanoparticles. Morphology study and cell viability, trypan blue exclusion, Annexin V-FITC/PI apoptosis and ROS generation assays showed that the FSNPs showed low toxicity to living cells. The FSNPs worked as fluorescent visualizers for selective imaging of the cytoplasm of tumor cells with over-expressed biotin receptors. The fluorescent nanoparticles were lastingly retained inside the living cells, thus enabling long-term tumor cell tracking over multiple passages and quantitative analysis of tumor cell migration.

引用

页码：676 / 684

页数：9