LC-MS/MS analysis of chitooligosaccharides

被引：15

作者：

Kim, Jaehyun ^{[1
]}

Kim, Jinhee ^{[1
]}

Hong, Jangmi ^{[1
]}

Lee, Sunyoung ^{[1
]}

Park, Sehwan ^{[1
]}

Lee, Ji-Hye ^{[1
]}

Kim, Jeongkwon ^{[1
]}

机构：

[1] Chungnam Natl Univ, Dept Chem, 220 Gung Dong, Taejon 305764, South Korea

来源：

CARBOHYDRATE RESEARCH | 2013年 / 372卷

关键词：

Chitooligosaccharides; HPLC; ESI; Mass spectrometry; Chitosan; N-ACETYLCHITOOLIGOSACCHARIDES; MEMBRANE REACTOR; CHITOSAN; CHITIN; CHROMATOGRAPHY; HYDROLYSIS; OLIGOMERS; MATRIX; AID;

D O I：

10.1016/j.carres.2013.02.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitooligosaccharides with degrees of polymerization (DPs) 2-12 were successfully separated and characterized using LC-MS/MS analysis with an amine column and a quadrupole ion trap analyzer. Major MS peaks for each chromatographic peak originated from deacetylated chitooligosaccharides while N-acetylated chitooligosaccharides were observed as minor peaks. The ratio of acetylation per glucosamine unit for each chitooligomer was higher for oligomers with higher DPs. The MS/MS spectra of chitooligosaccharides were used to characterize the chitooligosaccharide fragmentation patterns. To the best of our knowledge, this is the first report of on-line LC-MS/MS analysis of chitooligosaccharides with DP higher than 6. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：23 / 29

页数：7

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