LC-MS/MS analysis of chitooligosaccharides

被引：15
作者：
Kim, Jaehyun [1 ]
Kim, Jinhee [1 ]
Hong, Jangmi [1 ]
Lee, Sunyoung [1 ]
Park, Sehwan [1 ]
Lee, Ji-Hye [1 ]
Kim, Jeongkwon [1 ]
机构：
[1] Chungnam Natl Univ, Dept Chem, 220 Gung Dong, Taejon 305764, South Korea
来源：
CARBOHYDRATE RESEARCH | 2013年 / 372卷
关键词：
Chitooligosaccharides; HPLC; ESI; Mass spectrometry; Chitosan; N-ACETYLCHITOOLIGOSACCHARIDES; MEMBRANE REACTOR; CHITOSAN; CHITIN; CHROMATOGRAPHY; HYDROLYSIS; OLIGOMERS; MATRIX; AID;
D O I：
10.1016/j.carres.2013.02.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chitooligosaccharides with degrees of polymerization (DPs) 2-12 were successfully separated and characterized using LC-MS/MS analysis with an amine column and a quadrupole ion trap analyzer. Major MS peaks for each chromatographic peak originated from deacetylated chitooligosaccharides while N-acetylated chitooligosaccharides were observed as minor peaks. The ratio of acetylation per glucosamine unit for each chitooligomer was higher for oligomers with higher DPs. The MS/MS spectra of chitooligosaccharides were used to characterize the chitooligosaccharide fragmentation patterns. To the best of our knowledge, this is the first report of on-line LC-MS/MS analysis of chitooligosaccharides with DP higher than 6. (C) 2013 Elsevier Ltd. All rights reserved.
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页码：23 / 29
页数：7
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