Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield

被引：89

作者：

Guo, Mei ^{[1
]}

Rupe, Mary A. ^{[1
]}

Wei, Jun ^{[1
]}

Winkler, Chris ^{[1
]}

Goncalves-Butruille, Marymar ^{[1
]}

Weers, Ben P. ^{[1
]}

Cerwick, Sharon F. ^{[1
]}

Dieter, Jo Ann ^{[1
]}

Duncan, Keith E. ^{[2
]}

Howard, Richard J. ^{[2
]}

Hou, Zhenglin ^{[1
]}

Loeffler, Carlos M. ^{[1
]}

Cooper, Mark ^{[1
]}

Simmons, Carl R. ^{[1
]}

机构：

[1] DuPont Pioneer, Johnston, IA 50131 USA

[2] DuPont Pioneer, Wilmington, DE 19803 USA

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2014年 / 65卷 / 01期

关键词：

Maize hybrid; native allele variation; transgene by environment interaction; transgene efficacy; heterosis; ZAR1; Zea mays; GENE-EXPRESSION; ENVIRONMENTAL COVARIABLES; TOMATO FRUIT; ORGAN SIZE; SELECTION; PLANT; CROP; QTL; IDENTIFICATION; BIOTECHNOLOGY;

D O I：

10.1093/jxb/ert370

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Crop improvement for yield and drought tolerance is challenging due to the complex genetic nature of these traits and environmental dependencies. This study reports that transgenic over-expression of Zea mays ARGOS1 (ZAR1) enhanced maize organ growth, grain yield, and drought-stress tolerance. The ZAR1 transgene exhibited environmental interactions, with yield increase under Temperate Dry and yield reduction under Temperate Humid or High Latitude environments. Native ZAR1 allele variation associated with drought-stress tolerance. Two founder alleles identified in the mid-maturity germplasm of North America now predominate in Pioneers modern breeding programme, and have distinct proteins, promoters and expression patterns. These two major alleles show heterotic group partitioning, with one predominant in Pioneers female and the other in the male heterotic groups, respectively. These two alleles also associate with favourable crop performance when heterozygous. Allele-specific transgene testing showed that, of the two alleles discussed here, each allele differed in their impact on yield and environmental interactions. Moreover, when transgenically stacked together the allelic pair showed yield and environmental performance advantages over either single allele, resembling heterosis effects. This work demonstrates differences in transgenic efficacy of native alleles and the differences reflect their association with hybrid breeding performance.

引用

页码：249 / 260

页数：12

共 44 条

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