Molecular epidemiology of avian influenza virus and incidence of H5 and H9 virus subtypes among poultry in Egypt in 2009-2011

被引：4
作者：
Osman, N. [1 ]
Sultan, S. [2 ]
Ahmed, A. I. [1 ]
Ibrahim, R. S. [3 ]
El-Wanes, S. A. Abd [4 ]
Ibrahim, E. M. [5 ]
机构：
[1] South Valley Univ, Fac Vet Med, Dept Poultry Dis, Qena 83523, Egypt
[2] South Valley Univ, Fac Vet Med, Dept Microbiol Virol, Qena 83523, Egypt
[3] Assiut Univ, Fac Vet Med, Dept Poultry Dis, Assiut 71515, Egypt
[4] Cent Lab Vet Qual Control Poultry Prod CLQP, Cairo, Egypt
[5] Natl Lab Vet Qual Control Poultry Prod NLQP, Luxor, Egypt
来源：
ACTA VIROLOGICA | 2015年 / 59卷 / 01期
关键词：
avian influenza; H5; H9; subtyping; real-time RT-PCR; poultry; Egypt; A VIRUSES; HUMAN INFECTION; SOUTHERN CHINA; DOMESTIC DUCKS; GULF-COAST; HONG-KONG; EVOLUTION; PATHOGENICITY; PREVALENCE; WATERFOWL;
D O I：
10.4149/av_2015_01_27
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Egypt has experienced outbreaks of avian influenza (Al) since 2006. A total of 3583 cloacal swabs were collected from chickens, ducks, geese and turkeys from commercial farms, backyards and local bird markets in Qena and Luxor governorates in South Egypt during 2009-2011. These samples were examined for the presence of Al virus (AIV) and positive samples were further subtyped for the H5 and H9 by real time RT-PCR. In this way, 202 (5.64%) samples were found to be AIV-positive of which 186 (92.08%) and 7(3.46%) belonged to H5 and H9 subtypes, respectively. Higher infection rates were observed in backyard birds and birds from local bird markets in comparison to birds from commercial farms. In conclusion, the predominance of H5 infection indicates a need for continuous monitoring of AIV among avian species and the awareness against public health risk.
引用
收藏
页码：27 / 32
页数：6
相关论文
共 37 条
[11]   The challenge of avian influenza to the veterinary community [J].
Capua, Ilaria ;
Alexander, Dennis J. .
AVIAN PATHOLOGY, 2006, 35 (03) :189-205
[12]   The evolution of H5N1 influenza viruses in ducks in southern China [J].
Chen, H ;
Deng, G ;
Li, Z ;
Tian, G ;
Li, Y ;
Jiao, P ;
Zhang, L ;
Liu, Z ;
Webster, RG ;
Yu, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (28) :10452-10457
[13]  
Cox N.J., 2000, Virus Taxonomy, P585
[14]   The highly pathogenic avian influenza A (H7N7) virus epidemic in the Netherlands in 2003 - Lessons learned from the first five outbreaks [J].
Elbers, ARW ;
Fabri, THF ;
de Vries, TS ;
de Wit, JJ ;
Pijpers, A ;
Koch, G .
AVIAN DISEASES, 2004, 48 (03) :691-705
[15]   Avian influenza surveillance in hunter-harvested waterfowl from the Gulf Coast of Texas (November 2005-January 2006) [J].
Ferro, Pamela J. ;
El-Attrache, John ;
Fang, Xingwang ;
Rollo, Susan N. ;
Jester, Adam ;
Merendino, Todd ;
Peterson, Markus J. ;
Lupiani, Blanca .
JOURNAL OF WILDLIFE DISEASES, 2008, 44 (02) :434-439
[16]   Comparison of Real-Time Reverse Transcription-PCR and Virus Isolation for Estimating Prevalence of Avian Influenza Virus in Hunter-Harvested Wild Birds at Waterfowl Wintering Grounds Along the Texas Mid-Gulf Coast (2005-2006 Through 2008-2009) [J].
Ferro, Pamela J. ;
Peterson, Markus J. ;
Merendino, Todd ;
Nelson, Matt ;
Lupiani, Blanca .
AVIAN DISEASES, 2010, 54 (01) :655-659
[17]  
Food and Agricultural Organization, 2010, H5N1 HPAI GLOB OV JU
[18]   Epidemiology, production losses, and control measures associated with an outbreak of avian influenza subtype H7N2 in Pennsylvania (1996-98) [J].
Henzler, DJ ;
Kradel, DC ;
Davison, S ;
Ziegler, AF ;
Singletary, D ;
DeBok, P ;
Castro, AE ;
Lu, H ;
Eckroade, R ;
Swayne, D ;
Lagoda, W ;
Schmucker, B ;
Nesselrodt, A .
AVIAN DISEASES, 2003, 47 :1022-1036
[19]   Universal primer set for the full-length amplification of all influenza A viruses [J].
Hoffmann, E ;
Stech, J ;
Guan, Y ;
Webster, RG ;
Perez, DR .
ARCHIVES OF VIROLOGY, 2001, 146 (12) :2275-2289
[20]   Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia [J].
Hulse-Post, DJ ;
Sturm-Ramirez, KM ;
Humberd, J ;
Seiler, P ;
Govorkova, EA ;
Krauss, S ;
Scholtissek, C ;
Puthavathana, P ;
Buranathai, C ;
Nguyen, TD ;
Long, HT ;
Naipospos, TSP ;
Chen, H ;
Ellis, TM ;
Guan, Y ;
Peiris, JSM ;
Webster, RG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (30) :10682-10687
1234