Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase

被引：16

作者：

Dinamarco, Taisa Magnani ^{[3
]}

Brown, Neil Andrew ^{[1
]}

Couto de Almeida, Ricardo Sergio ^{[2
]}

de Castro, Patricia Alves ^{[1
]}

Savoldi, Marcela ^{[1
]}

de Souza Goldman, Maria Helena ^{[3
]}

Goldman, Gustavo Henrique ^{[1
,4
]}

机构：

[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo, Brazil

[2] Univ Londrina, Dept Microbiol, Londrina, Parana, Brazil

[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo, Brazil

[4] CTBE, Lab Nacl Ciencia & Tecnol Bioetanol, BR-13083970 Campinas, SP, Brazil

来源：

MICROBES AND INFECTION | 2012年 / 14卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

Aspergillus fumigatus; Calcineurin; Nucleoside diphosphate kinase; Galleria mellonella; NEUROSPORA-CRASSA; STRESS; FUNGI; NIDULANS; POLARITY; DEFECTS; RAM;

D O I：

10.1016/j.micinf.2012.05.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：922 / 929

页数：8

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