Rapid dipicolinic acid extraction from bacillus spores detected by surface-enhanced Raman spectroscopy

被引：53
作者：
Farquharson, S [1 ]
Gift, AD [1 ]
Maksymiuk, P [1 ]
Inscore, FE [1 ]
机构：
[1] Real Time Analyzers Ins, E Hartford, CT 06108 USA
来源：
APPLIED SPECTROSCOPY | 2004年 / 58卷 / 03期
关键词：
dipicolinic acid; Bacillus spores; anthrax; surface-enhanced; Raman spectroscopy;
D O I：
10.1366/000370204322886735
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
The rapid extraction of dipicolinic acid (DPA) from bacillus cereus spores aided by surface-enhanced Raman spectroscopy was investigated. The method is useful in detecting location of anthrax endospores in mail distribution facilities. The ability of this method to distinguish between spore-forming bacteria and non-DPA containing powders also helps prevent costly shutdowns associated with the appearance of suspicious materials. The results showed that DPA can be extracted from a 10μg bacillus cereus spore sample using DDA in 1 minute and can be detected by SERS in an additional 1 minute.
引用
收藏
页码：351 / 354
页数：4
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