Caveolin-1 expression in ovarian carcinoma is MDR1 independent

被引：0
作者：
Davidson, B
Goldberg, I
Givant-Horwitz, V
Nesland, JM
Berner, A
Bryne, M
Risberg, B
Kopolovic, J
Kristensen, GB
Tropé, CG
van de Putte, G
Reich, R
机构：
[1] Norwegian Radium Hosp, Dept Pathol, N-0310 Oslo, Norway
[2] Norwegian Radium Hosp, Dept Gynecol Oncol, N-0310 Oslo, Norway
[3] Univ Oslo, Dept Oral Biol, N-0316 Oslo, Norway
[4] Hebrew Univ Jerusalem, Fac Med, Dept Pharmacol, IL-91905 Jerusalem, Israel
[5] Hebrew Univ Jerusalem, David R Bloom Ctr Pharm, IL-91905 Jerusalem, Israel
[6] Chaim Sheba Med Ctr, Dept Pathol, IL-52621 Tel Hashomer, Israel
[7] Tel Aviv Univ, Sackler Fac Med, IL-69978 Tel Aviv, Israel
来源：
AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2002年 / 117卷 / 02期
关键词：
serous effusions; immunohistochemistry; mRNA in situ hybridization; reverse transcription-polymerase chain reaction; caveolin; multidrug resistance;
D O I：
暂无
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for camolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.
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收藏
页码：225 / 234
页数：10
相关论文
共 44 条
[1]   CAVEOLAE - WHERE INCOMING AND OUTGOING MESSENGERS MEET [J].
ANDERSON, RGW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :10909-10913
[2]   Expression of metastasis-related genes in surgical specimens of human gastric cancer can predict disease recurrence [J].
Anzai, H ;
Kitadai, Y ;
Bucana, CD ;
Sanchez, R ;
Omoto, R ;
Fidler, IJ .
EUROPEAN JOURNAL OF CANCER, 1998, 34 (04) :558-565
[3]   Downmodulation of caveolin-1 expression in human ovarian carcinoma is directly related to α-folate receptor overexpression [J].
Bagnoli, M ;
Tomassetti, A ;
Figini, M ;
Flati, S ;
Dolo, V ;
Canevari, S ;
Miotti, S .
ONCOGENE, 2000, 19 (41) :4754-4763
[4]  
Bedrossian CWM, 1994, Malignant Effusions: A Multimodal Approach to Cytologic Diagnosis
[5]   Expression of CD44 in effusions of patients diagnosed with serous ovarian carcinoma - diagnostic and prognostic implications [J].
Berner, HS ;
Davidson, B ;
Berner, A ;
Risberg, B ;
Kristensen, GB ;
Trope, CG ;
Van de Putte, G ;
Nesland, JM .
CLINICAL & EXPERIMENTAL METASTASIS, 2000, 18 (02) :197-202
[6]  
Bjorge T, 1998, INT J CANCER, V75, P663, DOI 10.1002/(SICI)1097-0215(19980302)75:5<663::AID-IJC1>3.3.CO
[7]  
2-4
[8]  
BOURHIS J, 1989, CANCER RES, V49, P5062
[9]   Clinical relevance of transmembrane drug efflux as a mechanism of multidrug resistance [J].
Bradshaw, DM ;
Arceci, RJ .
JOURNAL OF CLINICAL ONCOLOGY, 1998, 16 (11) :3674-3690
[10]   Interaction of a receptor tyrosine kinase, EGF-R, with caveolins - Caveolin binding negatively regulates tyrosine and serine/threonine kinase activities [J].
Couet, J ;
Sargiacomo, M ;
Lisanti, MP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (48) :30429-30438
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