Microfluidic probe for single-cell analysis in adherent tissue culture

被引:74
|
作者
Sarkar, Aniruddh [1 ]
Kolitz, Sarah [2 ]
Lauffenburger, Douglas A. [2 ]
Han, Jongyoon [1 ,2 ]
机构
[1] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA
来源
NATURE COMMUNICATIONS | 2014年 / 5卷
关键词
PROTEIN-KINASE ACTIVITY; SENESCENCE; PHOSPHORYLATION; VARIABILITY; METASTASIS; TUMOR; ACTIVATION; REPORTERS; APOPTOSIS; PATHWAYS;
D O I
10.1038/ncomms4421
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Single-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.
引用
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页数:8
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