Pinpointing Double Bonds in Lipids by Paterno-Buchi Reactions and Mass Spectrometry

被引：271

作者：

Ma, Xiaoxiao ^{[1
]}

Xia, Yu ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 10期

基金：

美国国家科学基金会;

关键词：

double bond location; Patern-Buchi reaction; photochemical reactions; tandem mass spectrometry; unsaturated lipids; OZONE-INDUCED DISSOCIATION; UNSATURATED FATTY-ACIDS; ELECTROSPRAY-IONIZATION; THERMAL-DECOMPOSITION; SHOTGUN LIPIDOMICS; POSITION; ESTERS; DERIVATIVES; DIFFERENTIATION; LOCATION;

D O I：

10.1002/anie.201310699

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The positions of double bonds in lipids play critical roles in their biochemical and biophysical properties. In this study, by coupling Patern-Buchi (P-B) reaction with tandem mass spectrometry, we developed a novel method that can achieve confident, fast, and sensitive determination of double bond locations within various types of lipids. The P-B reaction is facilitated by UV irradiation of a nanoelectrospray plume entraining lipids and acetone. Tandem mass spectrometry of the on-line reaction products via collision activation leads to the rupture of oxetane rings and the formation of diagnostic ions specific to the double bond location.

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页码：2592 / 2596

页数：5

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