Sensitive determination of pyrrolizidine alkaloids in Tussilago farfara L. by field-amplified, sample-stacking, sweeping micellar electrokinetic chromatography

被引:18
作者
Cao, Kun [1 ]
Xu, Yi [1 ,2 ,3 ]
Mu, Xiuni [1 ]
Zhang, Qing [1 ]
Wang, Renjie [1 ]
Lv, Junjiang [1 ]
机构
[1] Chongqing Univ, Sch Chem & Chem Engn, Chongqing, Peoples R China
[2] Def Key Disciplines Lab Novel Micronano Devices &, Chongqing, Peoples R China
[3] Int R&D Ctr Micronano Syst & New Mat Technol, Chongqing, Peoples R China
基金
中国国家自然科学基金;
关键词
Micellar electrokinetic capillary chromatography; Pyrrolizidine alkaloids; Organic acid esters; Tussilago farfara L; LIQUID-LIQUID MICROEXTRACTION; DYNAMIC PH JUNCTION; CAPILLARY-ELECTROPHORESIS; QUANTITATIVE-ANALYSIS; FLOWER BUDS; EXTRACTION; SEPARATION; PRODUCTS; WATER; PRECONCENTRATION;
D O I
10.1002/jssc.201600762
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Pyrrolizidine alkaloids are the toxic components in Tussilago farfara L. Due to the lack of standard substances for quantitative analysis and traces of pyrrolizidine alkaloids in total alkaloids, the full quality control of Tussilago farfara L has been limited. In this study, we aimed to solve the difficulty of determination of pyrrolizidine alkaloids and identify more components in the total alkaloids. An on-line preconcentration method has been applied to improve determining sensitivity of pyrrolizidine alkaloids in Tussilago farfara L. in which included field-amplified sample stacking and sweeping in micellar electrokinetic capillary chromatography. The main parameters that affected separation and stacking efficiency were investigated in details. Under the optimal conditions, the sensitivity enhancement factors obtained by the developed method for the analytes were from 15- to 12-fold, the limits of detection of senkirkine and senecionine were 2 similar to 5 mu g/L. Senkirkine and senecionine have been detected in alkaloids (c) of Tussilago farfara L, along ferulic acid methyl ester and methyl caffeate. The developed method was also applied to the analysis of acid extraction (a) of Tussilago farfara L, and senkirkine could be detected directly. The results indicated that the developed method is feasible for the analysis of pyrrolizidine alkaloids in Tussilago farfara L with good recoveries.
引用
收藏
页码:4243 / 4250
页数:8
相关论文
共 31 条
[1]  
[曹坤 Cao Kun], 2014, [天然产物研究与开发, Natural Product Research and Development], V26, P619
[2]   Safety Assessment of Food and Herbal Products Containing Hepatotoxic Pyrrolizidine Alkaloids: Interlaboratory Consistency and the Importance of N-oxide Determination [J].
Cao, Yu ;
Colegate, Steven M. ;
Edgar, John A. .
PHYTOCHEMICAL ANALYSIS, 2008, 19 (06) :526-533
[3]  
Chinese Pharmacopoeia Commission, 2010, PHARMACOPOEIA PEOPLE, V1, P316
[4]   Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence [J].
Gorman, BA ;
Barnett, NW ;
Bos, R .
ANALYTICA CHIMICA ACTA, 2005, 541 (1-2) :119-124
[5]   Determination of senkirkine and senecionine in Tussilago farfara using microwave-assisted extraction and pressurized hot water extraction with liquid chromatography tandem mass spectrometry [J].
Jiang, Zhangjian ;
Liu, Feng ;
Goh, Jennifer Jia Lei ;
Yu, Lijun ;
Li, Sam Fong Yau ;
Ong, Eng Shi ;
Ong, Choon Nam .
TALANTA, 2009, 79 (02) :539-546
[6]   Online sample pre-concentration via dynamic pH junction in capillary and microchip electrophoresis [J].
Kazarian, Artaches A. ;
Hilder, Emily F. ;
Breadmore, Michael C. .
JOURNAL OF SEPARATION SCIENCE, 2011, 34 (20) :2800-2821
[7]   Antioxidative effects of quercetin-glyco sides isolated from the flower buds of Tussilago farfara L. [J].
Kim, Mi-Ran ;
Lee, Jeong Yong ;
Lee, Hyang-Hee ;
Aryal, Dipendra Kuma ;
Kim, Yoon Gyoon ;
Kim, Sang Kyum ;
Woo, Eun-Rhan ;
Kang, Keon Wook .
FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (08) :1299-1307
[8]   Interface-free two-dimensional heart-cutting capillary electrophoresis for the separation and stacking of anionic and neutral analytes [J].
Kukusamude, Chunyapuk ;
Srijaranai, Supalax ;
Quirino, Joselito P. .
JOURNAL OF SEPARATION SCIENCE, 2015, 38 (14) :2532-2537
[9]  
Lebada R, 2000, PHYTOCHEM ANALYSIS, V11, P366, DOI 10.1002/1099-1565(200011/12)11:6<366::AID-PCA538>3.0.CO
[10]  
2-1