Functional analysis of female gametophyte specific promoters in Chinese cabbage

被引：1
作者：
Kim, Soo-Yun [1 ]
Yu, Hee-Ju [2 ]
Hong, Joon Ki [3 ]
Woo, Jong Gyu [1 ]
Ahn, Yul Kyun [1 ]
机构：
[1] Natl Inst Hort & Herbal Sci, RDA, Vegetable Res Div, Suwon 440706, South Korea
[2] Catholic Univ Korea, Dept Life Sci, Buxheon 420743, South Korea
[3] Rural Dev Adm, Natl Acad Agr Sci, Suwon 441707, South Korea
来源：
SCIENTIA HORTICULTURAE | 2013年 / 156卷
关键词：
Embryo sac; At5g40260; Chinese cabbage; Transformation; GUS; BRA0029160; Cis-element; SEED-SPECIFIC EXPRESSION; CIS-REGULATORY ELEMENTS; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; BRASSICA-RAPA; PEA LECTIN; CELL; L; IDENTIFICATION; TRANSFORMATION;
D O I：
10.1016/j.scienta.2013.03.003
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Female gametophyte, also called embryo sacs, plays an important role during double fertilization in angiosperm. The objective of this study was to develop specific promoters for female gametophyte to induce new fertility systems and protect varieties. Upstream region of At5g40260 gene, characterized female gametophyte specific expression gene in Arabidopsis, and upstream region of BRA0029160 gene in Chinese cabbage, homologous gene of At5g40260, were investigated to develop female gametophyte specific promoters. GUS reporter genes, which was driven by these two upstream regions of the gene (promoters), were detected in embryo sac and central vacuole. However, promoter of At5g40260 gene drove stronger expression than promoter of BRA0029160 gene in transgenic Chinese cabbage. Histochemical analysis revealed promoter of At5g40260 gene drove GUS expression in inner integument of division tissue of embryo sac in T-1 generation. Enzyme activity assay showed GUS gene expression in the embryo. Analysis of At5g40260 and BRA0029160 promoters by PlantCARE database revealed many cis-elements regulated initiation and modulation of the gene expression. (C) 2013 Elsevier B.V. All rights reserved.
引用
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页码：29 / 37
页数：9
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