Red Cabbage (Brassica oleracea) as a New Source of High-Thermostable Peroxidase

被引：15

作者：

Fortea, M. I. ^{[2
]}

Pellicer, J. A. ^{[1
]}

Serrano-Martinez, A. ^{[1
]}

Lopez-Miranda, S. ^{[1
]}

Lucas-Abellan, C. ^{[1
]}

Nunez-Delicado, E. ^{[1
]}

机构：

[1] Univ Catolica San Antonio Murcia UCAM, Dept Ciencia & Tecnol Alimentos, Murcia 30107, Spain

[2] Univ Catolica San Antonio Murcia UCAM, Dept Enfermeria, Murcia 30107, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 42期

关键词：

Peroxidase; kinetic parameters; thermal inactivation; SDS; reducing agent; cyclodextrin; LATENT POLYPHENOL OXIDASE; KINETIC CHARACTERIZATION; THERMAL INACTIVATION; PARTIAL-PURIFICATION; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; PHASE-SEPARATION; CANCER RISK; L; CYCLODEXTRINS;

D O I：

10.1021/jf302483q

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Soluble and membrane-bound peroxidases (PODs) were extracted from red cabbage using Triton X-114. Optimum activity was obtained at pH 4.0 for both enzymes, and both were inactivated by sodium dodecyl sulfate (SDS). The K-M and V-m values for H2O2 were found to be 0.98 mM and 8.1 mu M/min, respectively, for soluble POD and 0.82 mM and 6.1 mu M/min, respectively, for membrane-bound POD. When the 2,2'-azinobis(3-ethylbenzothiazolinesulfonic acid (ABTS) concentration was increased, maintaining a steady concentration of H2O2, the activity was inhibited at the highest ABTS concentrations in soluble POD. Ascorbic acid was found to be the most active modulator of POD activity. The effect of cyclodextrins was also studied, and the complexation constant between ABTS and hydroxypropyl-beta-cyclodextrins (HP-beta-CDs) was calculated (K-c = 312 M-1). Membrane-bound POD is more thermostable than soluble POD, losing >90% of relative activity after 5 min of incubation at 76.6 and 30.2 degrees C, respectively.

引用

页码：10641 / 10648

页数：8

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