Promoter analysis of interleukin 19

被引：9

作者：

Chen, Po-Jen

Wei, Chi-Chen

Wang, Chihuei

Chen, Feng-Wei

Hsu, Yu-Hsiang

Chang, Ming-Shi ^{[1
]}

机构：

[1] Chi Mei Med Ctr, Tainan, Taiwan

[2] Natl Cheng Kung Univ, Inst Basic Med Sci, Tainan 70101, Taiwan

[3] Chung Hwa Coll Med Technol, Dept Med Technol, Tainan, Taiwan

[4] Kaohsiung Med Univ, Orthoped Res Ctr, Kaohsiung, Taiwan

[5] Kaohsiung Med Univ, Dept Biol, Kaohsiung, Taiwan

[6] Natl Cheng Kung Univ, Grad Inst Biochem & Mol Biol, Tainan 70101, Taiwan

[7] Natl Cheng Kung Univ, Inst Biopharmaceut Sci, Coll Med, Tainan 70101, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 03期

关键词：

interleukin-19; AMLI;

D O I：

10.1016/j.bbrc.2006.03.200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin (IL)-19 belongs to the IL-10 family which includes IL-19. IL-20, IL-22, AK155, and MDA-7. IL-10 is a potent immunomodulatory cytokine with implications for pathogenesis in various autoimmune diseases. Polymorphism of' the IL-10 promoter region correlates with disease outcome. To understand the gene regulation of IL-19, we analyzed the IL-19 promoter region. A regulatory region (PE). 148 bp upstream of exon I of IL-19 and linked to a luciferase reporter gene, supported luciferase activity 13 times greater than that supported by a negative promoterless control. An electrophoretic mobility shift assay (EMSA) showed specific binding sites for the transcription factors of the oligonucleotides PE1 (-148 to -98) derived from PE. We identified the sequence TGTGGT (-142 to -138) on PEI as the binding site for the transcription factor AML1, and crucial for the promoter activity of IL-19 because Substituting 1 bp in the PE region (-139G -> T) abolished IL-19 promoter activity. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：713 / 720

页数：8

共 17 条

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