Rapid capacitive detection of femtomolar levels of bisphenol A using an aptamer-modified disposable microelectrode array

被引：35

作者：

Cui, Haochen ^{[1
]}

Wu, Jayne ^{[1
]}

Eda, Shigetoshi ^{[2
]}

Chen, Jiangang ^{[3
]}

Chen, Wei ^{[4
]}

Zheng, Lei ^{[4
]}

机构：

[1] Univ Tennessee, Dept Elect Engn & Comp Sci, Knoxville, TN 37996 USA

[2] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA

[3] Univ Tennessee, Dept Publ Hlth, Knoxville, TN 37996 USA

[4] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China

来源：

MICROCHIMICA ACTA | 2015年 / 182卷 / 13-14期

关键词：

Capacitive sensing; Aptasensor; Bisphenol A; AC electrokinetics; IMMUNOSENSOR; FLUID; DIELECTROPHORESIS; ELECTROKINETICS; PARTICLES; BOTTLES; HPLC;

D O I：

10.1007/s00604-015-1556-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free and single-step method is reported for rapid and highly sensitive detection of bisphenol A (BPA) in aqueous samples. It utilizes an aptamer acting as a probe molecule immobilized on a commercially available array of interdigitated aluminum microelectrodes. BPA was quantified by measuring the interfacial capacitance change rate caused by the specific binding between bisphenol A and the immobilized aptamer. The AC signal also induces an AC electrokinetic effect to generate microfluidic motion for enhanced binding. The capacitive aptasensor achieves a limit of detection as low as 10 fM(2.8 fg a <...aEuro parts per thousand mL (-aEuro parts per thousand 1)) with a 20 s response time. The method is inexpensive, highly sensitive, rapid and therefore provides a promising technology for on-site detection of BPA in food and water samples. B. The ACEK capacitive aptasensor has a limit of detection as low as 10 fM (2.8 fg amL-(1)) with a 20-s response time.

引用

页码：2361 / 2367

页数：7

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