Polysaccharides from peach pulp: Structure and effects on mouse peritoneal macrophages

被引：43

作者：

Simas-Tosin, F. F. ^{[1
]}

Abud, A. P. R. ^{[2
]}

De Oliveira, C. C. ^{[2
]}

Gorin, P. A. J. ^{[1
]}

Sassaki, G. L. ^{[1
]}

Bucchi, D. F. ^{[2
]}

Iacomini, M. ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, PR, Brazil

[2] Univ Fed Parana, Dept Biol Celular, BR-81531980 Curitiba, PR, Brazil

来源：

FOOD CHEMISTRY | 2012年 / 134卷 / 04期

关键词：

Peach pulp; Polysaccharides; Immunostimulation; Macrophages; ANGICO BRANCO; ARABINOGALACTAN; PECTIN; ACIDS; GUM;

D O I：

10.1016/j.foodchem.2012.04.041

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Pulp from peaches contained polygalacturonic acid and arabinogalactan as main polysaccharides, which were isolated and characterised. The polygalacturonic acid (AE-CWI) contained 95% GalA and its C-13 NMR spectrum showed signals at delta 98.9, 78.0, 71.4, 69.1, 68.4, and 175.1 from C-1, C-4, C-5, C-3, C-2, and C-6 respectively, from (1 -> 4)-linked alpha-GalpA units. Methylation-MS analysis of carboxy-reduced material (AE-CWI-CR) gave 90% of 2,3,6-Me-3-galactitol acetate. The arabinogalactan (AE-AG) was composed mainly of Ara (41%) and Gal (50%) and was characterised (methylation analysis and C-13 NMR) as a type II-arabinogalactan. It induced peritoneal macrophage activation in mice, similar to 70% of cells treated with this fraction (1-50 mu g/mL) having morphology of activated cells. However, NO production in macrophages treated with AE-AG was not affected. This suggests a new biological activity for peach polysaccharides. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2257 / 2260

页数：4

共 27 条

[1] Determination of immunomodulatory effects: focus on functional analysis of phagocytes as representatives of the innate immune system [J].

Bilitewski, Ursula .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (05) :1545-1554

[2] Arginase modulates nitric oxide production in activated macrophages [J].

Chang, CI ;

Liao, JC ;

Kuo, L .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1998, 274 (01) :H342-H348

[3] A polysaccharide from a tea (infusion) of Maytenus ilicifolia leaves with anti-ulcer protective effects [J].

Cipriani, Thales R. ;

Mellinger, Caroline G. ;

de Souza, Lauro M. ;

Baggio, Cristiane H. ;

Freitas, Cristina S. ;

Marques, Maria C. A. ;

Gorin, Philip A. J. ;

Sassaki, Guilherme L. ;

Iacomini, Marcello .

JOURNAL OF NATURAL PRODUCTS, 2006, 69 (07) :1018-1021

[4]

CIUCANU I, 1984, J CHROMATOGR, V284, P179, DOI 10.1016/S0021-9673(01)87813-4

[5] The free reducing oligosaccharides of angico branco (Anadenanthera colubrina) gum exudate:: an aid for structural assignments in the heteropolysaccharide [J].

Delgobo, CL ;

Gorin, PAJ ;

Tischer, CA ;

Iacomini, M .

CARBOHYDRATE RESEARCH, 1999, 320 (3-4) :167-175

[6] MEASUREMENT OF URONIC-ACIDS WITHOUT INTERFERENCE FROM NEUTRAL SUGARS [J].

FILISETTICOZZI, TMCC ;

CARPITA, NC .

ANALYTICAL BIOCHEMISTRY, 1991, 197 (01) :157-162

[7] Alternative Activation of Macrophages: Mechanism and Functions [J].

Gordon, Siamon ;

Martinez, Fernando O. .

IMMUNITY, 2010, 32 (05) :593-604

[8] POLYSACCHARIDES OF THE LICHENS CETRARIA-ISLANDICA AND RAMALINA-USNEA [J].

GORIN, PAJ ;

IACOMINI, M .

CARBOHYDRATE RESEARCH, 1984, 128 (01) :119-132

[9] FURTHER STUDIES ON ASSIGNMENT OF SIGNALS IN C-13 MAGNETIC-RESONANCE SPECTRA OF ALDOSES AND DERIVED METHYL GLYCOSIDES [J].

GORIN, PAJ ;

MAZUREK, M .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1975, 53 (08) :1212-1223

[10] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

← 1 2 3 →