An Advanced In Vitro Technology Platform to Study the Mechanism of Action of Prebiotics and Probiotics in the Gastrointestinal Tract

被引:12
作者
Marzorati, Massimo [1 ]
Van de Wiele, Tom [1 ]
机构
[1] Univ Ghent, CMET, Coupure Links 653, B-9000 Ghent, Belgium
关键词
gut simulation; SHIME; microbiome; mucosal; INTESTINAL MICROBIAL ECOSYSTEM; FERMENTATION PROFILE; SIMULATOR; OLIGOSACCHARIDES; LACTOBACILLI; SHIME(R);
D O I
10.1097/MCG.0000000000000711
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The gastrointestinal tract (GIT) hosts the most complex microbial community in the human body. Given the extensive metabolic potential which is present in this community, this additional organ is of key importance to maintain a healthy status and several diseases are frequently correlated with an alteration of the composition/functionality of the gut microbiota. Consequently, there is a great interest in identifying potential approaches that could modulate the microbiota and its metabolism to bring about a positive health effect. A classical approach to reach this goal is the use of prebiotics and/or probiotics. How to study the potential effect of new prebiotics/probiotics and how to localize this effect along the full GIT? Human intervention trials are the golden standard to validate functional properties of food products. Yet, most studies on gut microbiota are based on the analysis of fecal samples because they are easily collected in a non-invasive manner. A complementary option is represented by well-designed in vitro simulation technologies. Among all the available systems, the Simulator of Human Intestinal Microbial Ecosystem has already been shown to be a useful model for nutrition studies in terms of analysis of the intestinal microbial community composition and activity. The Simulator of Human Intestinal Microbial Ecosystem is a scientifically validated platform representing the physiology and microbiology of the adult human GIT. Furthermore, recent advances in in vitro modelling also allow to combine the study of bacteria-host interactions, such as mucosal adhesion and interaction with the immune system, thereby further increasing the value of the scientific output.
引用
收藏
页码:S124 / S125
页数:2
相关论文
共 12 条
[1]   Diversity of the human intestinal microbial flora [J].
Eckburg, PB ;
Bik, EM ;
Bernstein, CN ;
Purdom, E ;
Dethlefsen, L ;
Sargent, M ;
Gill, SR ;
Nelson, KE ;
Relman, DA .
SCIENCE, 2005, 308 (5728) :1635-1638
[2]   Genes and Molecules of Lactobacilli Supporting Probiotic Action [J].
Lebeer, Sarah ;
Vanderleyden, Jos ;
De Keersmaecker, Sigrid C. J. .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2008, 72 (04) :728-+
[3]  
Macfarlane S, 2009, CRIT REV CL LAB SCI, V46, P25, DOI [10.1080/10408360802485792 , 10.1080/10408360802485792]
[4]   Addition of acacia gum to a FOS/inulin blend improves its fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) [J].
Marzorati, Massimo ;
Qin, Bingcai ;
Hildebrand, Falk ;
Klosterbuer, Abby ;
Roughead, Zamzam ;
Roessle, Claudia ;
Rochat, Florence ;
Raes, Jeroen ;
Possemiers, Sam .
JOURNAL OF FUNCTIONAL FOODS, 2015, 16 :211-222
[5]   The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro [J].
Marzorati, Massimo ;
Vanhoecke, Barbara ;
De Ryck, Tine ;
Sadabad, Mehdi Sadaghian ;
Pinheiro, Iris ;
Possemiers, Sam ;
Van den Abbeele, Pieter ;
Derycke, Lara ;
Bracke, Marc ;
Pieters, Jan ;
Hennebel, Tom ;
Harmsen, Hermie J. ;
Verstraete, Willy ;
Van de Wiele, Tom .
BMC MICROBIOLOGY, 2014, 14
[6]  
Marzorati M, 2009, AGRO FOOD IND HI TEC, V20, P50
[7]   A Dried Yeast Fermentate Selectively Modulates both the Luminal and Mucosal Gut Microbiota and Protects against Inflammation, As Studied in an Integrated in Vitro Approach [J].
Possemiers, Sam ;
Pinheiro, Iris ;
Verhelst, An ;
Van den Abbeele, Pieter ;
Maignien, Lois ;
Laukens, Debby ;
Reeves, Stuart G. ;
Robinson, Larry E. ;
Raas, Thomas ;
Schneider, Yves-Jacques ;
Van de Wiele, Tom ;
Marzorati, Massimo .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (39) :9380-9392
[8]   Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem [J].
Sanchez, J. I. ;
Marzorati, M. ;
Grootaert, C. ;
Baran, M. ;
Van Craeyveld, V. ;
Courtin, C. M. ;
Broekaert, W. F. ;
Delcour, J. A. ;
Verstraete, W. ;
Van de Wiele, T. .
MICROBIAL BIOTECHNOLOGY, 2009, 2 (01) :101-113
[9]   Arabinogalactan and fructo-oligosaccharides have a different fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) [J].
Terpend, Kathleen ;
Possemiers, Sam ;
Daguet, David ;
Marzorati, Massimo .
ENVIRONMENTAL MICROBIOLOGY REPORTS, 2013, 5 (04) :595-603
[10]   The Human Microbiome Project [J].
Turnbaugh, Peter J. ;
Ley, Ruth E. ;
Hamady, Micah ;
Fraser-Liggett, Claire M. ;
Knight, Rob ;
Gordon, Jeffrey I. .
NATURE, 2007, 449 (7164) :804-810