Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2

被引：20

作者：

Jang, Seonghui ^{[1
]}

Shin, Heegwon ^{[1
]}

Lee, Jungmin ^{[1
]}

Kim, Youngmi ^{[1
]}

Bak, Geunu ^{[1
]}

Lee, Younghoon ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem, 291 Daehak Ro, Daejeon 34141, South Korea

来源：

FEBS LETTERS | 2017年 / 591卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

BC200; RNA; hnRNP E1; hnRNP E2; regulation of translation; RNA-protein interaction; LONG NONCODING RNA; DENDRITIC BC1 RNA; MESSENGER-RNA; BINDING-PROTEINS; ERYTHROID-DIFFERENTIATION; POLY(C)-BINDING PROTEINS; POLY(A)-BINDING PROTEIN; GENE-EXPRESSION; POLIOVIRUS RNA; BREAST-CANCER;

D O I：

10.1002/1873-3468.12544

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The long noncoding RNA BC200 ( brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA-inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 30 C-rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA-mediated translation inhibition.

引用

页码：393 / 405

页数：13

共 49 条

[41]

Sosinska P, 2015, GINEKOL POL, V86, P782

[42] PRIMARY STRUCTURE, NEURAL-SPECIFIC EXPRESSION, AND DENDRITIC LOCATION OF HUMAN BC200 RNA [J].

TIEDGE, H ;

CHEN, W ;

BROSIUS, J .

JOURNAL OF NEUROSCIENCE, 1993, 13 (06) :2382-2390

[43] Structure and function of KH domains [J].

Valverde, Roberto ;

Edwards, Laura ;

Regan, Lynne .

FEBS JOURNAL, 2008, 275 (11) :2712-2726

[44]

Wang HD, 2002, J NEUROSCI, V22, P10232

[45] HnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression [J].

Woolaway, Kathryn ;

Asai, Kengo ;

Emili, Andrew ;

Cochrane, Alan .

RETROVIROLOGY, 2007, 4

[46] Downregulation of BC200 in ovarian cancer contributes to cancer cell proliferation and chemoresistance to carboplatin [J].

Wu, Di ;

Wang, Tianzhen ;

Ren, Chengcheng ;

Liu, Lei ;

Kong, Dan ;

Jin, Xiaoming ;

Li, Xiaobo ;

Zhang, Guangmei .

ONCOLOGY LETTERS, 2016, 11 (02) :1189-1194

[47] Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif [J].

Zalfa, F ;

Adinolfi, S ;

Napoli, I ;

Kühn-Hölsken, E ;

Urlaub, H ;

Achsel, T ;

Pastore, A ;

Bagni, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (39) :33403-33410

[48] BC200 LncRNA a potential predictive marker of poor prognosis in esophageal squamous cell carcinoma patients [J].

Zhao, Rui-Hua ;

Zhu, Cai-Hua ;

Li, Xiang-Ke ;

Cao, Wei ;

Zong, Hong ;

Cao, Xin-Guang ;

Hu, Hai-Yan .

ONCOTARGETS AND THERAPY, 2016, 9 :2221-2226

[49] Expression of poly(C)-binding proteins is differentially regulated by hypoxia and ischemia in cortical neurons [J].

Zhu, Y ;

Sun, Y ;

Mao, XO ;

Jin, KL ;

Greenberg, DA .

NEUROSCIENCE, 2002, 110 (02) :191-198

← 1 2 3 4 5 →