Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2

被引：20

作者：

Jang, Seonghui ^{[1
]}

Shin, Heegwon ^{[1
]}

Lee, Jungmin ^{[1
]}

Kim, Youngmi ^{[1
]}

Bak, Geunu ^{[1
]}

Lee, Younghoon ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem, 291 Daehak Ro, Daejeon 34141, South Korea

来源：

FEBS LETTERS | 2017年 / 591卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

BC200; RNA; hnRNP E1; hnRNP E2; regulation of translation; RNA-protein interaction; LONG NONCODING RNA; DENDRITIC BC1 RNA; MESSENGER-RNA; BINDING-PROTEINS; ERYTHROID-DIFFERENTIATION; POLY(C)-BINDING PROTEINS; POLY(A)-BINDING PROTEIN; GENE-EXPRESSION; POLIOVIRUS RNA; BREAST-CANCER;

D O I：

10.1002/1873-3468.12544

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The long noncoding RNA BC200 ( brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA-inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 30 C-rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA-mediated translation inhibition.

引用

页码：393 / 405

页数：13

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