Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils

被引：305

作者：

Felske, A ^{[1
]}

Rheims, H ^{[1
]}

Wolterink, A ^{[1
]}

Stackebrandt, E ^{[1
]}

Akkermans, ADL ^{[1
]}

机构：

[1] DSM RES BV,DEUTSCH SAMMLUNG MIKROORGANISMEN & ZELLKULTUREN,D-38124 BRAUNSCHWEIG,GERMANY

来源：

MICROBIOLOGY-UK | 1997年 / 143卷

关键词：

16S rDNA library; Actinobacteria; temperature-gradient gel electrophoresis (TGGE); ribosome extraction from soil; V6; probe;

D O I：

10.1099/00221287-143-9-2983

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 16S rRNA-based molecular ecological study was performed to search for dominant bacterial sequences in Drentse A grassland soils (The Netherlands). In the first step, a library of 165 clones was generated from PCR-amplified 165 rDNA. By sequence comparison, clone DA079 and two other identical clones could be affiliated to a group of recently described uncultured Actinobacteria. This group contained 165 rDNA clone sequences obtained from different ribosomes were isolated from the environment and 165 rRNA was partially amplified via RT-PCR using conserved primers for members of the domain Bacteria. Subsequent sequence-specific separation by temperature-gradient gel electrophoresis (TGGE) generated fingerprints of the amplicons. Such community fingerprints were compared with the TGGE pattern of PCR-amplified rDNA of clone DA079 which was generated with the same set of primers. One of the dominant fingerprint bands matched with the band obtained from the actinobacterial clone. Southern blot hybridization with a probe made from clone DA079 confirmed sequence identity of clone and fingerprint band. This is the first report that a member of the novel actinobacterial group may play a physiologically active role in a native microbial community.

引用

页码：2983 / 2989

页数：7

共 33 条

[1] THE RELATIONSHIP BETWEEN CELL-SIZE AND VIABILITY OF SOIL BACTERIA [J].

BAKKEN, LR ;

OLSEN, RA .

MICROBIAL ECOLOGY, 1987, 13 (02) :103-114

[2] Molecular microbial diversity of an agricultural soil in Wisconsin [J].

Borneman, J ;

Skroch, PW ;

OSullivan, KM ;

Palus, JA ;

Rumjanek, NG ;

Jansen, JL ;

Nienhuis, J ;

Triplett, EW .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (06) :1935-1943

[3]

BROSIUS J, 1978, P NATL ACAD SCI USA, V75, P4801, DOI 10.1073/pnas.75.10.4801

[4] Acidimicrobium ferrooxidans gen nov, sp nov: Mixed-culture ferrous iron oxidation with Sulfobacillus species [J].

Clark, DA ;

Norris, PR .

MICROBIOLOGY-UK, 1996, 142 :785-790

[5]

Engelen B, 1995, WORKSH APPL DGGE TGG

[6]

Felsenstein J, 1993, PHYLIP (Phylogeny Inference Package) version 3.5c

[7] Direct ribosome isolation from soil to extract bacterial rRNA for community analysis [J].

Felske, A ;

Engelen, B ;

Nubel, U ;

Backhaus, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) :4162-4167

[8] PHYLOGENETIC DIVERSITY OF SUBSURFACE MARINE MICROBIAL COMMUNITIES FROM THE ATLANTIC AND PACIFIC OCEANS [J].

FUHRMAN, JA ;

MCCALLUM, K ;

DAVIS, AA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) :1294-1302

[9]

HARTUNG K, 1996, THESIS FACHHOCHSCHUL

[10]

Heuer H., 1997, Modern soil microbiology., P353

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