Evaluation of genetic variation in high molecular weight glutenin subunits of seed storage protein using landraces of common wheat from Pakistan

被引:3
作者
Niwa, K. [1 ]
Suzuki, H. [1 ]
Tominaga, T. [2 ]
Nasim, S. [3 ]
Anwar, R. [3 ]
Ogawa, M. [4 ]
Furuta, Y. [5 ]
机构
[1] Tokyo Univ Agr, Dept Agr, Atsugi, Kanagawa 2430034, Japan
[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068502, Japan
[3] Natl Agr Res Ctr, Plant Genet Resources Inst, Islamabad 45500, Pakistan
[4] Yamaguchi Prefectural Univ, Dept Human Environm, Yamaguchi 7538502, Japan
[5] Gifu Univ, Fac Appl Biol Sci, Gifu 5011193, Japan
关键词
common wheat; glutenin; HMW-GS; Pakistan;
D O I
10.1556/CRC.36.2008.2.12
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
SDS-PAGE was used to determine the variation in high molecular weight glutenin subunits (HMW-GS) alleles present in 170 landraces of common wheat from Sindh, Balochistan and NWFP in Pakistan. Three alleles for Glu-A1, six alleles for Glu-B1 and four alleles for Glu-D1 were detected. These included three new alleles, namely Glu-B1e, Glu-B1h and Glu-D1n, which had not previously been found in landraces of Pakistani wheat. Furthermore, Glu-D1m and Glu-D1n, which are considered to be endemic alleles in Pakistan and Afghanistan, were both detected from Balochistan. Twenty genotypes were identified based on combinations of alleles at the three Glu-1 loci. The present study showed that Pakistani landraces of common wheat have maintained a broad diversity of HMW-GS alleles, and that they may serve as genetic resources to improve wheat varieties adapted to Pakistani climatic and edaphic conditions.
引用
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页码:327 / 332
页数:6
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