Detection and Genetic Characterization of Lymphocystis Disease Virus (LCDV) Isolated during Disease Outbreaks in Cultured Gilt-head Sea Bream Sparus aurata in Tunisia

被引:0
|
作者
Haddad-Boubaker, Sondes [1 ]
Bouzgarou, Nadia [2 ]
Fakhfakh, Emna [1 ]
Khayech, Monia [2 ]
Ben Mohamed, Souhail [2 ]
Megdich, Aida [1 ]
Ben Chehida, Noureddine [1 ]
机构
[1] Vet Res Inst Tunisia, Virol Lab, Tunis 1006, Tunisia
[2] Reg Ctr Vet Res Sousse, Sousse 4000, Tunisia
来源
FISH PATHOLOGY | 2013年 / 48卷 / 03期
关键词
Sprarus aurata; lymphocystis disease virus; phylogeny; aquaculture; Tunisia; MAJOR CAPSID PROTEIN; SEABREAM; GENOTYPE; SKIN; SOLE;
D O I
暂无
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Lymphocystis disease virus (LCDV) causes a self-limiting disease in fish. However, recently, LCD outbreaks have increased, causing significant mortalities. In this study, we report the occurrence of two disease outbreaks in reared gilt-head sea bream Sparus aurata in the Tunisian coast. Presence of LCDV in diseased fish was confirmed by virus isolation using BF-2 cells and PCR amplification targeting the polymerase gene. Amino acid sequence analysis of the major capsid protein suggested that the LCDV strain was identical to nine strains previously isolated from the Mediterranean, Red Sea and South Atlantic coasts of Europe. These strains might have been disseminated through recent international trade.
引用
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页码:101 / 104
页数:4
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