Defective CD3ζ chain expression in Herpesvirus saimiri (HVS)-derived T-cell lines in gastric adenocarcinoma

被引:9
作者
Lopez-Santalla, M
Krishnan, S
Valeri, AP
Aguilera-Montilla, N
Fisher, CU
Perez-Blas, M
Gutierrez-Calvo, A
Lasa, I
Granell-Vicent, J
Tsokos, GC
Martin-Villa, JM [1 ]
机构
[1] Univ Complutense Madrid, Fac Med, E-28040 Madrid, Spain
[2] Hosp Univ Principe Asturias, Serv Cirugia Gen & Aparato Digest, Alcala De Henares, Spain
[3] Walter Reed Army Inst Res, Silver Spring, MD 20910 USA
关键词
CD3; zeta; T lymphocytes; gastric adenocarcinoma;
D O I
10.1016/j.cellimm.2006.02.007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Low expression of the CD3 zeta chain has been reported in patients with cancer and it has been suggested that tumor-derived factors are involved in its downregulation. The expression of CD3 zeta chain was measured in T-cell lines from patients with gastric adenocarcinoma and healthy volunteers and grown in vitro for several months and, hence, in the absence of any tumor-derived factors. T-cell lines of mucosal origin were obtained by Herpesvirus saimiri transformation from gastric cancer patients. The expression of CD3 zeta and CD3 epsilon was measured by flow cytometry and Western-blot analysis. Calcium mobilization and apoptosis rate were also measured. The levels of CD3 zeta, but not CD3 epsilon, chain on the cell surface were significantly reduced in T-cell lines derived from patients with gastric cancer when cultured in the absence of IL-2. Western-blot analysis of total cell extracts or lipid raft fractions confirmed this finding. Calcium mobilization, a measure of signal transduction, was reduced in T cell lines from patients with gastric cancer. We conclude that T cells from patients with cancer express lower levels of CD3 zeta. This downregulation is not caused by a direct effect of tumor-derived factors but, rather, it appears to be inherent to the patient cells. The low CD3 zeta expression would render T lymphocytes unable to control the growth of tumor cells. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:113 / 122
页数:10
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