Green gram husk -: an inexpensive substrate for alkaline protease production by Bacillus sp in solid-state fermentation

被引：187

作者：

Prakasham, RS ^{[1
]}

Rao, CS ^{[1
]}

Sarma, PN ^{[1
]}

机构：

[1] Indian Inst Chem Technol, Biochem & Environm Engn Ctr, Hyderabad 500007, Andhra Pradesh, India

来源：

BIORESOURCE TECHNOLOGY | 2006年 / 97卷 / 13期

关键词：

alkaline protease; Bacillus sp; enzyme; green gram husk; solid-state fermentation;

D O I：

10.1016/j.biortech.2005.07.015

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Alkaline protease production under solid-state fermentation was investigated using isolated alkalophilic Bacillus sp. Among all agro-industrial waste material evaluated, green gram husk supported maximum protease production. Solid material particle size regulated the enzyme production and yield was improved with the supplementation of carbon and nitrogen sources to the solid medium. Optimum enzyme production was achieved with 1.5% maltose and 2.0% yeast extract with 371% increase than control. Glucose did not repressed enzyme production but inorganic nitrogen sources showed little negative impact. The physiological fermentation factors such as pH of the medium (pH 9.0), moisture content (140%), incubation time (60 h) and inoculum level played a vital role in alkaline protease production. The enzyme production was found to be associated with the growth of the bacterial culture. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1449 / 1454

页数：6

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