Label-Free Detection of Tumor Markers in a Colon Carcinoma Tumor Progression Model by Confocal Raman Microspectroscopy

被引：1

作者：

Scalfi-Happ, Claudia ^{[1
]}

Rueck, Angelika ^{[1
]}

Udart, Martin ^{[1
]}

Hauser, Carmen ^{[1
]}

Duerr, Christine

Kriebel, Martin

机构：

[1] Inst Laser Technol Med & Metrol ILM, D-89081 Ulm, Germany

来源：

CLINICAL AND BIOMEDICAL SPECTROSCOPY AND IMAGING III | 2013年 / 8798卷

关键词：

Raman microscopy; confocal microscopy; optical diagnostics for medicine; living cells analysis; gastrointestinal; OPTICAL TECHNIQUES; SPECTROSCOPY; DIAGNOSIS;

D O I：

10.1117/12.2032459

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Living colon carcinoma cells were investigated by confocal Raman microspectroscopy. An in vitro model of tumor progression was established. Evaluation of data sets by cluster analysis reveals that lipid bodies might be a valuable diagnostic parameter for early carcinogenesis.

引用

页数：6

共 16 条

[1] Lipid bodies are reservoirs of cyclooxygenase-2 and sites of prostaglandin-E2 synthesis in colon cancer cells [J].

Accioly, Maria T. ;

Pacheco, Patricia ;

Maya-Monteiro, Clarissa M. ;

Carrossini, Nina ;

Robbs, Bruno K. ;

Oliveira, Silvia S. ;

Kaufmann, Cristiane ;

Morgado-Diaz, Jose A. ;

Bozza, Patricia T. ;

Viola, Joao P. B. .

CANCER RESEARCH, 2008, 68 (06) :1732-1740

[2] Endoscopic imaging: emerging optical techniques for the detection of colorectal neoplasia [J].

Anandasabapathy, Sharmila .

CURRENT OPINION IN GASTROENTEROLOGY, 2008, 24 (01) :64-69

[3]

[Anonymous], 2013, COLORECTAL CANC, P2011

[4] Lipid droplets: A dynamic organelle moves into focus [J].

Beller, Mathias ;

Thiel, Katharina ;

Thul, Peter J. ;

Jaeckle, Herbert .

FEBS LETTERS, 2010, 584 (11) :2176-2182

[5] Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells [J].

Chen, K ;

Qin, YJ ;

Zheng, F ;

Sun, MH ;

Shi, DR .

OPTICS LETTERS, 2006, 31 (13) :2015-2017

[6] Discrimination of normal and malignant mucosal tissues of the colon by Raman Spectroscopy [J].

Chowdary, M. V. P. ;

Kumar, K. Kalyan ;

Thakur, Keerthi ;

Anand, A. ;

Kurien, Jacob ;

Krishna, C. Murali ;

Mathew, Stanley .

PHOTOMEDICINE AND LASER SURGERY, 2007, 25 (04) :269-274

[7]

DaCosta RS, 2005, CURR OPIN GASTROEN, V21, P70

[8] Lessons from hereditary colorectal cancer [J].

Kinzler, KW ;

Vogelstein, B .

CELL, 1996, 87 (02) :159-170

[9] Studies on stress-induced changes at the subcellular level by Raman microspectroscopic mapping [J].

Krafft, C ;

Knetschke, T ;

Funk, RHW ;

Salzer, R .

ANALYTICAL CHEMISTRY, 2006, 78 (13) :4424-4429

[10] Raman and CARS microspectroscopy of cells and tissues [J].

Krafft, Christoph ;

Dietzek, Benjamin ;

Popp, Juergen .

ANALYST, 2009, 134 (06) :1046-1057

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