Development of Bacillus subtilis mutants to produce tryptophan in pigs

被引：11

作者：

Bjerre, Karin ^{[1
]}

Cantor, Mette D. ^{[1
]}

Norgaard, Jan V. ^{[2
]}

Poulsen, Hanne D. ^{[2
]}

Blaabjerg, Karoline ^{[2
]}

Canibe, Nuria ^{[2
]}

Jensen, Bent B. ^{[2
]}

Stuer-Lauridsen, Birgitte ^{[1
]}

Nielsen, Bea ^{[1
]}

Derkx, Patrick M. F. ^{[1
]}

机构：

[1] Chr Hansen AS, Boge Alle 10-12, DK-2970 Horsholm, Denmark

[2] Aarhus Univ, Dept Anim Sci, DK-8830 Tjele, Denmark

来源：

BIOTECHNOLOGY LETTERS | 2017年 / 39卷 / 02期

关键词：

Bacillus subtilis; mtrB; trpS; Tryptophan; UV-mutagenesis; BINDING ATTENUATION PROTEIN; PERFORMANCE; TRAP; ACID;

D O I：

10.1007/s10529-016-2245-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To generate tryptophan-overproducing Bacillus subtilis strains for in situ use in pigs, to reduce the feed cost for farmers and nitrogen pollution. A novel concept has been investigated-to generate B. subtilis strains able to produce tryptophan (Trp) in situ in pigs. Mutagenesis by UV was combined with selection on Trp and purine analogues in an iterative process. Two mutants from different wild types were obtained, mutant 1 (M1) produced 1 mg Trp/l and mutant 2 (M2) 14 mg Trp/l. Genome sequence analysis revealed that M1 had three single nuclear polymorphisms (SNPs) and M2 had two SNPs compared to the wild type strains. In both mutants SNPs were found in genes regulating tryptophan synthesis. Reverse transcription PCR confirmed up-regulation of the tryptophan synthesis genes in both mutants, the expression was up to 3 times higher in M2 than in M1. Tryptophan-excreting B. subtilis strains were obtained with UV-mutagenesis and analogue selection and can be used in animal feed applications.

引用

页码：289 / 295

页数：7

共 17 条

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