Isolation, purification, and properties of α-amylase from Bacillus subtilis-7A

被引：3
作者：
Nurmatov, SK [1 ]
Akhmedova, ZR
Rakhimov, DA
机构：
[1] Acad Sci Uzbek, Inst Microbiol, Tashkent, Uzbekistan
[2] Acad Sci Uzbek, S Yu Yunusov Inst Chem Plant Subst, Tashkent, Uzbekistan
来源：
CHEMISTRY OF NATURAL COMPOUNDS | 2001年 / 37卷 / 04期
关键词：
Bacillus subtilis; alpha-amylase; neutral protease; complex preparation; isolation; purification; properties; amylolytic and proteolytic activity; specificity;
D O I：
10.1023/A:1013782804567
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Cultivation of Bacillus subtilis-7A on waste from alcohol production yielded an active extracellular enzyme alpha-amylase with MW 75 kDa. The enzyme was isolated from the culture medium by 60% saturated ammonium sulfate and purified until homogeneous by gel filtration on Sephadex G-100 and ion-exchange chromatography on DEAE-cellulose. The optimum temperatures for the complex and purified enzyme are 30 and 50degreesC, respectively. The optimum activity for both preparations occurred at pH 6.5. The substrate specificity of the isolated preparations was studied.
引用
收藏
页码：364 / 368
页数：5
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