A direct fluorescence-based technique for cellular localization of amylin

被引：1

作者：

Pillay, Karen ^{[1
]}

Govender, Patrick ^{[1
]}

机构：

[1] Univ KwaZulu Natal, Sch Life Sci, ZA-4000 Durban, South Africa

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2013年 / 60卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

amylin; carboxyfluorescein; RIN-5F; type II diabetes; ISLET AMYLOID POLYPEPTIDE; PEPTIDE-BASED INHIBITORS; FIBRIL FORMATION; DIABETES-MELLITUS; AMINO-ACID; BETA; MEMBRANE; TOXICITY; IAPP; DYSFUNCTION;

D O I：

10.1002/bab.1113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amylin has been implicated in type II diabetes because of its inherent property to misfold into toxic aggregates. Although it has been shown that amylin interacts with cell membranes, no study to date has monitored the association process using a direct approach. The present study uses confocal microscopy to identify the localization of carboxyfluorescein-labeled amylin in RIN-5F cells. In addition, the size of the aggregates that are formed was evaluated using nanoparticle tracking analysis. In support of previous findings, amylin was observed to interact with and remain associated with the cell membrane. The cell membrane-associated aggregates spanned a size range of 130-800nm.

引用

页码：384 / 392

页数：9

共 50 条

[1] A novel fluorescence-based cellular permeability assay
Chandra, Ankur
Barillas, Samuel
Suliman, Ahmed
Angle, Niren
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2007, 70 (03): : 329 - 333
[2] Development of a fluorescence-based cellular apoptosis reporter
Balderstone, Lucy A.
Dawson, John C.
Welman, Arkadiusz
Serrels, Alan
Wedge, Stephen R.
Brunton, Valerie G.
METHODS AND APPLICATIONS IN FLUORESCENCE, 2019, 7 (01):
[3] Direct fluorescence-based lipase activity assay
Diaz, P
Prim, N
Pastor, FIJ
BIOTECHNIQUES, 1999, 27 (04) : 696 - +
[4] Fluorescence-based techniques for the investigation of localization and functions of proteins
Chang, Yuen-Yan
Lai, Yau-Tsz
Hu, Ligang
Yang, Ya
Chao, Ailun
Sun, Hongzhe
PROTEIN SCIENCE, 2015, 24 : 4 - 5
[5] Fluorescence-based protocol for revealing cellular arrangement in biofilms
Puri, Devina
Fang, Xin
Allison, Kyle R.
STAR PROTOCOLS, 2023, 4 (02):
[6] Fluorescence-Based Detection and Quantification of Features of Cellular Senescence
Cho, Sohee
Hwang, Eun Seong
RECENT ADVANCES IN CYTOMETRY, PART B: ADVANCES IN APPLICATIONS, FIFTH EDITION, 2011, 103 : 149 - 188
[7] Novel fluorescence-based assays for cellular viability and cytotoxicity
Buller, GM
Haugland, RP
Haugland, RP
CYTOMETRY, 2002, : 78 - 78
[8] Fluorescence-Based In Situ Quantitative Imaging for Cellular Lipids
Cho, W.
Yoon, Y.
Liu, S. -L.
Baek, K.
Sheng, R.
ENZYMOLOGY AT THE MEMBRANE INTERFACE: INTERFACIAL ENZYMOLOGY AND PROTEIN-MEMBRANE BINDING, 2017, 583 : 19 - 33
[9] Direct fluorescence-based detection methods for multivalent interactions
Song, XD
Shi, J
Swanson, BI
RECOGNITION OF CARBOHYDRATES IN BIOLOGICAL SYSTEMS PT A: GENERAL PROCEDURES, 2003, 362 : 523 - 542
[10] Filtering technique for fluorescence-based DNA sequencing data
Domnisoru, C
Musavi, M
2000 CANADIAN CONFERENCE ON ELECTRICAL AND COMPUTER ENGINEERING, CONFERENCE PROCEEDINGS, VOLS 1 AND 2: NAVIGATING TO A NEW ERA, 2000, : 489 - 493

← 1 2 3 4 5 →