Genome-wide identification and expression analysis of calcium-dependent protein kinase and its closely related kinase genes in Capsicum annuum

被引:44
|
作者
Cai, Hanyang [1 ,2 ]
Cheng, Junbin [1 ,2 ]
Yan, Yan [1 ,3 ]
Xiao, Zhuoli [1 ,2 ]
Li, Jiazhi [1 ,2 ]
Mou, Shaoliang [1 ,2 ]
Qiu, Ailian [1 ,2 ]
Lai, Yan [1 ,2 ]
Guan, Deyi [1 ,3 ]
He, Shuilin [1 ,3 ]
机构
[1] Fujian Agr & Forestry Univ, Natl Educ Minist, Key Lab Plant Genet Improvement & Comprehens Util, Fuzhou 350002, Fujian, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China
[3] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Fujian, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2015年 / 6卷
基金
中国国家自然科学基金;
关键词
CDPKs; CRKs; Capsicum annuum; salt; heat; and R. solanacearum; ARABIDOPSIS RESPONSES; PROVIDES INSIGHTS; STRESS TOLERANCE; CONFERS SALT; RICE; CDPK; EVOLUTION; DROUGHT; TOMATO; FAMILY;
D O I
10.3389/fpls.2015.00737
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
As Ca2+ sensors and effectors, calcium-dependent protein kinases (CDPKs) play important roles in plant growth, development, and response to environmental cues. However, no CDPKs have been characterized in Capsicum annuum thus far. Herein, a genome wide comprehensive analysis of genes encoding CDPKs and CDPK-related protein kinases (CRKs) was performed in pepper, a total of 31 CDPK genes and five closely related kinase genes were identified, which were phylogenetically divided into four distinct subfamilies and unevenly distributed across nine chromosomes. Conserved sequence and exon-intron structures were found to be shared by pepper CDPKs within the same subfamily, and the expansion of the CDPK family in pepper was found to be due to segmental duplication events. Five CDPKs in the C. annuum variety CM334 were found to be mutated in the Chiltepin variety, and one CDPK present in CM334 was lost in Chiltepin. The majority of CDPK and CRK genes were expressed in different pepper tissues and developmental stages, and 10, 12, and 8 CDPK genes were transcriptionally modified by salt, heat, and Ralstonia solanacearum stresses, respectively. Furthermore, these genes were found to respond specifically to one stress as well as respond synergistically to two stresses or three stresses, suggesting that these CDPK genes might be involved in the specific or synergistic response of pepper to salt, heat, and R. solanacearum. Our results lay the foundation for future functional characterization of pepper CDPK and its closely related gene families.
引用
收藏
页数:14
相关论文
共 50 条
  • [21] Genome-Wide Identification and Characterization of Calcium-Dependent Protein Kinase (CDPK) and CDPK-Related Kinase (CRK) Gene Families in Medicago truncatula
    Zhao, Pengcheng
    Liu, Yajie
    Kong, Weiyi
    Ji, Jiayi
    Cai, Tianyu
    Guo, Zhenfei
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (03) : 1 - 16
  • [22] Genome-wide identification of the calcium-dependent protein kinase gene family in Fragaria vesca and expression analysis under different biotic stresses
    Yifan Xiong
    Dahe Lin
    Shiwei Ma
    Chunhua Wang
    Shoukai Lin
    European Journal of Plant Pathology, 2022, 164 : 283 - 298
  • [23] Genome-wide identification of the calcium-dependent protein kinase gene family in Fragaria vesca and expression analysis under different biotic stresses
    Xiong, Yifan
    Lin, Dahe
    Ma, Shiwei
    Wang, Chunhua
    Lin, Shoukai
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2022, 164 (02) : 283 - 298
  • [24] Biochemical and expression analysis of an Arabidopsis calcium-dependent protein kinase-related kinase
    Du, W
    Wang, Y
    Liang, SP
    Lu, YT
    PLANT SCIENCE, 2005, 168 (05) : 1181 - 1192
  • [25] Genome-Wide Survey Indicates Diverse Physiological Roles of Dendrobium officinale Calcium-Dependent Protein Kinase Genes
    Yang, Xingyu
    Chen, Zhiyu
    Yin, Xin
    Wang, Yuhua
    Yang, Yunqiang
    Yang, Yongping
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (03)
  • [26] Calcium-dependent protein kinase (CDPK) and CDPK-related kinase (CRK) gene families in tomato: genome-wide identification and functional analyses in disease resistance
    Ji-Peng Wang
    You-Ping Xu
    Jean-Pierre Munyampundu
    Tian-Yu Liu
    Xin-Zhong Cai
    Molecular Genetics and Genomics, 2016, 291 : 661 - 676
  • [27] Genome-wide identification and expression analysis of Raf-like kinase gene family in pepper (Capsicum annuum L.)
    Lim, Chae Woo
    Lee, Sung Chul
    PLANT SIGNALING & BEHAVIOR, 2022, 17 (01)
  • [28] Calcium-dependent protein kinase (CDPK) and CDPK-related kinase (CRK) gene families in tomato: genome-wide identification and functional analyses in disease resistance
    Wang, Ji-Peng
    Xu, You-Ping
    Munyampundu, Jean-Pierre
    Liu, Tian-Yu
    Cai, Xin-Zhong
    MOLECULAR GENETICS AND GENOMICS, 2016, 291 (02) : 661 - 676
  • [29] Genome-wide identification and expression analysis of salinity stress-related protein kinase in cotton
    Rahman, Shafeeq Ur
    Shehzad, Muhammad
    Qin, Anzhen
    Zain, Muhammad
    Shahzad, Sher Muhammad
    Ansari, Mohammad Javed
    Alharbi, Sulaiman Ali
    AGRONOMY JOURNAL, 2024, 116 (03) : 1302 - 1320
  • [30] Genome-Wide Identification and Analysis of Expression Profiles of Maize Mitogen-Activated Protein Kinase Kinase Kinase
    Kong, Xiangpei
    Lv, Wei
    Zhang, Dan
    Jiang, Shanshan
    Zhang, Shizhong
    Li, Dequan
    PLOS ONE, 2013, 8 (02):