Optical inhibition of motor nerve and muscle activity in vivo

被引：30

作者：

Liske, Holly ^{[1
]}

Towne, Chris ^{[2
]}

Anikeeva, Polina ^{[3
]}

Zhao, Shengli ^{[4
]}

Feng, Guoping ^{[5
]}

Deisseroth, Karl ^{[2
,6
]}

Delp, Scott ^{[1
,2
]}

机构：

[1] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[3] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[4] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA

[5] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA

[6] Stanford Univ, Dept Psychiat & Behav Sci, Stanford, CA 94305 USA

来源：

MUSCLE & NERVE | 2013年 / 47卷 / 06期

基金：

美国国家卫生研究院;

关键词：

electromyography; halorhodopsin; optogenetics; sciatic nerve; spastic hypertonia; GENE-THERAPY; SPASTIC HYPERTONIA; CONDUCTION BLOCK; NEURAL CIRCUITRY; VECTOR; INTERROGATION; HALORHODOPSIN; TRANSDUCTION; STIMULATION; TECHNOLOGY;

D O I：

10.1002/mus.23696

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Introduction: There is no therapeutic approach that provides precise and rapidly reversible inhibition of motor nerve and muscle activity for treatment of spastic hypertonia. Methods: We used optogenetics to demonstrate precise and rapidly reversible light-mediated inhibition of motor nerve and muscle activity in vivo in transgenic Thy1::eNpHR2.0 mice. Results: We found optical inhibition of motor nerve and muscle activity to be effective at all muscle force amplitudes and determined that muscle activity can be modulated by changing light pulse duration and light power density. Conclusions: This demonstration of optical inhibition of motor nerves is an important advancement toward novel optogenetics-based therapies for spastic hypertonia. Muscle Nerve 47: 916921, 2013

引用

页码：916 / 921

页数：6

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