An update on the prevalence of glucose-6-phosphate dehydrogenase deficiency and neonatal jaundice in Tehran neonates

Objectives: The aim of this study was to screen newborns in Tehran for glucose-6-phosphate dehydrogenase (G6PD) deficiency in relation to hyperbilirubinemia and jaundice. Design and methods: We performed quantitative and qualitative red blood cell (RBC) G6PD assays in cord blood of 2000 male and female at-term neonates. Observations for jaundice and bilirubin determination were made in G6PD-deficient and normal groups. Those with severe jaundice were treated with phototherapy or exchange transfusion. Results: Our results showed that 2.1% (3.6% of males and 0.6% of females) was G6PD-deficient. Those with severe jaundice and hyperbilirubinemia (160 normal and 17 G6PD-deficient) were hospitalized and treated with phototherapy or exchange transfusion. Bilirubin levels in G6PD-deficient neonates were somewhat higher compared to G6PD-nonnal babies (18.8 +/- 2.4 mg/dl [321.5 +/- 41 mumol/l] vs. 15.7 +/- 3.2 mg/dl [268.5 +/- 54.7 mumol/l]; P < 0.05). G6PD activity was significantly lower in G6PD-deficient group than in the normal group (2.1 +/- 0.7 vs. 12.5 +/- 5.0 U/g Hb; P < 0.001). Conclusion: This study shows that the incidence of G6PD deficiency in newborns of Tehran is 2.1 %, which is relatively high, and also hyperbilirubinemia and jaundice are approximately 3-fold higher in G6PD-deficients than in the G6PD-normal group (5 1 % vs. 16%). This emphasizes the necessity of neonatal screening on cord blood samples of both sexes for G6PD deficiency and the need to watch closely for development of hyperbilirubinemia. (C) 2003 The Canadian Society of Clinical Chemists. All rights reserved.