Monitoring oxygen concentration during photodynamic therapy using prompt photosensitizer fluorescence

被引：22
作者：
Weston, Mark A. [1 ]
Patterson, Michael S. [2 ]
机构：
[1] Juravinski Canc Ctr, Hamilton, ON L8V 5C2, Canada
[2] McMaster Univ, Dept Med Phys & Appl Radiat Sci, Hamilton, ON L8S 4L8, Canada
来源：
PHYSICS IN MEDICINE AND BIOLOGY | 2013年 / 58卷 / 20期
关键词：
DERIVATIVE MONOACID RING; SINGLET OXYGEN; IN-VIVO; OPTICAL-PROPERTIES; LUMINESCENCE; PDT; DOSIMETRY; PROSTATE; LIGHT; MODEL;
D O I：
10.1088/0031-9155/58/20/7039
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A novel technique is described that uses either time-resolved or steady state prompt photosensitizer fluorescence to measure local oxygen concentration. Solution experiments conducted with Al(III) phthalocyanine chloride tetrasulfonic acid confirmed that the steady state fluorescence signal is dependent on the oxygen concentration and fluence rate. A relationship between prompt sensitizer fluorescence and sensitizer triplet quenching efficiency is derived which does not require knowledge of the Stern-Volmer constant. Similar relationships are also derived for sensitizer delayed fluorescence and phosphorescence. An explicit photodynamic therapy (PDT) dose metric that incorporates light dosimetry, sensitizer dosimetry, and triplet quenching efficiency is introduced. All components of this metric can be determined by optical measurements.
引用
收藏
页码：7039 / 7059
页数：21
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